CD3 antibody

Catalog No. ABIN2689164

There are 3+ publications for this product available. The Rat Monoclonal anti-CD3 antibody is suitable to detect CD3 in samples from Mouse. It has been validated for FACS, IP, IHC (fro) and CyTox.

Quick Overview for CD3 antibody (ABIN2689164)

Target: CD3

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox)

Clone: 17A2

Product Details anti-CD3 Antibody

Characteristics: The 17A2 antibody reacts with the T-cell receptor-associated CD3 complex, which is expressed on many thymocytes and mature T lymphocytes. Plate-bound mAb 17A2 has been reported to induce IL-2 production by T cells in the absence of accessory cells. Binding of 17A2 antibody to T cells can be blocked by the anti-CD3e mAb 145-2C11, suggesting that 17A2 antibody recognizes an epitope of the CD3 epsilon chain. In vivo treatment with 17A2 mAb has been reported to partially deplete T lymphocytes and temporarily down-modulates CD3 expression on T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. CD3 expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with purified mAb 17A2 (bottom left panel) and PE-conjugated anti-mouse CD19 mAb 1D3 (top left & bottom left panels), followed by FITC-conjugated anti-rat IgG2b mAb G15-337 (Cat. No. 553884) (top left & bottom left panels). BALB/c thymocytes were also stained with 17A2 mAb (bottom right panel), followed by FITC-conjugated anti-rat IgG2b (top right & bottom right panels). Flow cytometry was performed on a BD FACSCan™ flow cytometry system.

BD Pharmingen™ Purified Rat Anti-Mouse CD3 Molecular Complex - Purified - Clone 17A2 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: gammadelta TCR-positive T-T hybridoma D1

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD3 antibody (ABIN2689164)

Mysliwietz, Thierfelder et al.: "Antilymphocytic antibodies and marrow transplantation. XII. Suppression of graft-versus-host disease by T-cell-modulating and depleting antimouse CD3 antibody is most effective when preinjected in ..." in: Blood, Vol. 80, Issue 10, pp. 2661-7, (1992) (PubMed).

Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).

Miescher, Schreyer, MacDonald: "Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex." in: Immunology letters, Vol. 23, Issue 2, pp. 113-8, (1990) (PubMed).

Target Details for CD3

Target: CD3

Alternative Name: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway