CD31 antibody

Catalog No. ABIN2689181

This Mouse Monoclonal antibody specifically detects CD31 in FACS, IHC, ELISA, IHC (p), IP and BR. It exhibits reactivity toward Rat and Pig. It has been mentioned in 5+ publications

Quick Overview for CD31 antibody (ABIN2689181)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Rat, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Blocking Reagent (BR)

Clone: TLD

Product Details anti-CD31 Antibody

Characteristics: The TLD-3A12 antibody reacts with CD31, also known as PECAM-1 (platelet endothelial cell adhesion molecule). CD31 is a ~130 kDa integral membrane glycoprotein, a member of the immunoglobulin super family, that mediates homophilic and heterophilic cell-cell adhesion. CD31 is expressed on endothelial cells, platelets, and subsets of leukocytes. In the human and mouse, multiple alternatively spliced isoforms have been identified, and this alternative splicing may be involved in the regulation of ligand specificity. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis in the mouse and rat. In addition, PECAM-1 has been demonstrated to play an important role in extravasation of leukocytes in vivo, but in vivo treatment with TLD-3A12 mAb had no discernable effect upon the inflammatory infiltrate in experimental allergic encephalomyelitis. The TLD-3A12 mAb partially blocks the proliferation response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This mouse anti-rat CD31 antibody cross-reacts with pig endothelial cells and platelets, as determined by immunohistochemical staining of acetone-fixed frozen sections and immunofluorescent staining with flow cytometric analysis, respectively. A suspension of Lewis rat microglial cells were used as the source of the immunogen. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. The expression of CD31 on rat spleen leukocytes. LEW splenocytes were stained with purified mAb TLD-3A12 (right panel) followed by biotinylated anti-mouse Igg1 mAb A85-1 (Cat. No. 553441), then FITC-conjugated anti-rat CD45RA mAb OX-33 (Cat. No. 554883) and Streptavidin-PE (Cat. No. 554061). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD31 - Purified - Clone TLD-3A12 - Isotype Mouse IgG1, κ - Reactivity Rat, Pig - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Lewis Rat Microglia

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD31 antibody (ABIN2689181)

DeLisser, Christofidou-Solomidou, Strieter, Burdick, Robinson, Wexler, Kerr, Garlanda, Merwin, Madri, Albelda: "Involvement of endothelial PECAM-1/CD31 in angiogenesis." in: The American journal of pathology, Vol. 151, Issue 3, pp. 671-7, (1997) (PubMed).

Famiglietti, Sun, DeLisser, Albelda: "Tyrosine residue in exon 14 of the cytoplasmic domain of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) regulates ligand binding specificity." in: The Journal of cell biology, Vol. 138, Issue 6, pp. 1425-35, (1997) (PubMed).

Williams, Zhao, Ueno, Hickey: "PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat." in: Journal of neuroscience research, Vol. 45, Issue 6, pp. 747-57, (1997) (PubMed).

Wakelin, Sanz, Dewar, Albelda, Larkin, Boughton-Smith, Williams, Nourshargh: "An anti-platelet-endothelial cell adhesion molecule-1 antibody inhibits leukocyte extravasation from mesenteric microvessels in vivo by blocking the passage through the basement membrane." in: The Journal of experimental medicine, Vol. 184, Issue 1, pp. 229-39, (1996) (PubMed).

DeLisser, Newman, Albelda: "Molecular and functional aspects of PECAM-1/CD31." in: Immunology today, Vol. 15, Issue 10, pp. 490-5, (1994) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31

Background: Synonyms: PECAM-1

Pathways: Regulation of Actin Filament Polymerization