CD31 antibody

Catalog No. ABIN2689184

This Rat Monoclonal antibody specifically detects CD31 in FACS, IP, IHC (f), IHC (fro), BR and IHC (zinc). It exhibits reactivity toward Mouse. It has been mentioned in 8+ publications

Quick Overview for CD31 antibody (ABIN2689184)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD31 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: MEC 13-3

Product Details anti-CD31 Antibody

Characteristics: The MEC13.3 antibody reacts with CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). CD31 is a 130 kDa integral membrane protein, a member of the immunoglobulin superfamily, that mediates cell-to-cell adhesion. CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is weakly expressed on many peripheral leukocytes and platelets. It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells. CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues. Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development, this alternative splicing is involved in the regulation of ligand specificity. CD38 and vitronectin receptor (αvβ3 integrin, CD51/CD61) are proposed to be ligands for CD31. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis. The MEC13.3 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31. Immunohistochemical staining of endothelial cells. The zinc-fixed paraffin-embedded section of U-87 MG tumor in mouse brain was stained with MEC13.3 mAb. Note the brown labeling of endothelia of blood vessels in the tumor.

BD Pharmingen™ Purified Rat Anti-Mouse CD31 - Purified - Clone MEC 13.3 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: 129/Sv mouse-derived endothelioma cell line tEnd.1

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 15.625 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD31 antibody (ABIN2689184)

Peluso, Jang, Dräger, Schwob: "Differential expression of components of the retinoic acid signaling pathway in the adult mouse olfactory epithelium." in: The Journal of comparative neurology, Vol. 520, Issue 16, pp. 3707-26, (2013) (PubMed).

Yoshioka, Hisanaga, Kawano: "Suppression of fibrotic scar formation promotes axonal regeneration without disturbing blood-brain barrier repair and withdrawal of leukocytes after traumatic brain injury." in: The Journal of comparative neurology, Vol. 518, Issue 18, pp. 3867-81, (2010) (PubMed).

Mahoney, Benton, Maddie, Whittemore, Hagg: "ADAM8 is selectively up-regulated in endothelial cells and is associated with angiogenesis after spinal cord injury in adult mice." in: The Journal of comparative neurology, Vol. 512, Issue 2, pp. 243-55, (2009) (PubMed).

Benton, Maddie, Minnillo, Hagg, Whittemore: "Griffonia simplicifolia isolectin B4 identifies a specific subpopulation of angiogenic blood vessels following contusive spinal cord injury in the adult mouse." in: The Journal of comparative neurology, Vol. 507, Issue 1, pp. 1031-52, (2008) (PubMed).

Vanzulli, Gazzaniga, Braidot, Vecchi, Mantovani, Wainstok de Calmanovici: "Detection of endothelial cells by MEC 13.3 monoclonal antibody in mice mammary tumors." in: Biocell : official journal of the Sociedades Latinoamericanas de Microscopi?a Electronica ... et. al, Vol. 21, Issue 1, pp. 39-46, (1997) (PubMed).

DeLisser, Christofidou-Solomidou, Strieter, Burdick, Robinson, Wexler, Kerr, Garlanda, Merwin, Madri, Albelda: "Involvement of endothelial PECAM-1/CD31 in angiogenesis." in: The American journal of pathology, Vol. 151, Issue 3, pp. 671-7, (1997) (PubMed).

Piali, Hammel, Uherek, Bachmann, Gisler, Dunon, Imhof: "CD31/PECAM-1 is a ligand for alpha v beta 3 integrin involved in adhesion of leukocytes to endothelium." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 451-60, (1995) (PubMed).

DeLisser, Newman, Albelda: "Molecular and functional aspects of PECAM-1/CD31." in: Immunology today, Vol. 15, Issue 10, pp. 490-5, (1994) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31

Background: Synonyms: PECAM-1

Pathways: Regulation of Actin Filament Polymerization