EpCAM antibody

Catalog No. ABIN2689192

Product Details anti-EpCAM Antibody

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Brand: BD Pharmingen™

Characteristics: The G8.8 antibody reacts with CD326/Ep-CAM (Epithelial Cell Adhesion Molecule), also known as gp40 in the mouse and by a variety of names (including GA733-2, CO17-1A, and EGP) in the human. In the mouse, Ep-CAM is a 40-42 kDa cell-surface glycoprotein expressed on thymic epithelial cells, thymic dendritic cells, immature thymocytes, a small subset of peripheral T lymphocytes, intestinal epithelium, kidney-collecting tubule epithelium, keratinocytes, Langerhans cells and lymph node and splenic dendritic cells. Profiles of Ep-CAM expression on fetal thymocytes and on the CD4[-] CD8[-], CD4[+] CD8[+], CD4[-] CD8[+], and CD4[+] CD8[-] subsets of adult thymocytes have been published. In unrelated studies, mouse Ep-CAM mRNA was detected in tissues containing epithelial cells (kidney, stomach, intestine, lung, and thymus) and in plasma cells and plasmacytomas, but not in heart, muscle, liver, brain, spleen, B lymphomas, or pre-B lymphomas. Ep-CAM is a Ca[2+] independent homophilic adhesion molecule which is proposed to play roles in the development and normal function of epithelial tissues and in the progression of carcinomas. Multicolor analysis of the expression of Ep- CAM on splenic T lymphocytes. BALB/c splenocytes were stained with FITC Hamster Anti-Mouse CD3e (Cat. No 553061/553062), PE Rat Anti-Mouse CD25 (Cat. No. 553866), and either Purified Rat IgG2a, κ Isotype Control (Cat. No. 553927, Left Panel) or Purified Rat Anti-Mouse CD326 (Cat. No. 552370, Right Panel) in the presence of Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™, Cat. No. 553141/553142). The G8.8 and isotype control mAb were detected by Biotin Mouse anti-Rat IgG2a (Cat. No. 553894), followed by Streptavidin-APC (Cat. No. 554067). Viable lymphocytes were gated for exclusion of propidium iodide, and the CD25+ subpopulation was selected for analysis of CD3e and Ep-CAM expression. A small population of CD3+CD25+Ep-CAM+ cells were detected (Right Panel), whereas the CD25- T cells do not express Ep-CAM (data not shown). Flow cytometry was performed on a BD FACSCalibur™

BD Pharmingen™ Purified Rat Anti-Mouse CD326 - Purified - Clone G8.8 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Glycoconjugates from BALB/c mouse-derived TE-71 medullary thymic epithelial cell line

Clone: G8-8

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-EpCAM antibody (ABIN2689192)

Birebent, Somasundaram, Purev, Li, Mitchell, Hoey, Bloom, Mastrangelo, Maguire, Harris, Staib, Braumuller, Leeser, Kuttner, Beger, Herlyn: "Anti-idiotypic antibody and recombinant antigen vaccines in colorectal cancer patients." in: Critical reviews in oncology/hematology, Vol. 39, Issue 1-2, pp. 107-13, (2001) (PubMed).

Taguchi, Hashimoto, Naiki, Farr, Boyd, Ansari, Shultz, Kotzin, Dorshkind, Ikehara, Gershwin: "Abnormal thymic expression of epithelial cell adhesion molecule (EP-CAM) in New Zealand Black (NZB) mice." in: Journal of autoimmunity, Vol. 13, Issue 4, pp. 393-404, (2000) (PubMed).

Basak, Speicher, Eck, Wunner, Maul, Simmons, Herlyn: "Colorectal carcinoma invasion inhibition by CO17-1A/GA733 antigen and its murine homologue." in: Journal of the National Cancer Institute, Vol. 90, Issue 9, pp. 691-7, (1998) (PubMed).

Zutter: "Gastrointestinal carcinoma antigen GA733: target for immunodestruction and potential modifier of invasiveness and chemoresponsiveness." in: Journal of the National Cancer Institute, Vol. 90, Issue 9, pp. 642-4, (1998) (PubMed).

Litvinov, Balzar, Winter, Bakker, Briaire-de Bruijn, Prins, Fleuren, Warnaar: "Epithelial cell adhesion molecule (Ep-CAM) modulates cell-cell interactions mediated by classic cadherins." in: The Journal of cell biology, Vol. 139, Issue 5, pp. 1337-48, (1997) (PubMed).

Nelson, Dunn, Peach, Aruffo, Farr: "The murine homolog of human Ep-CAM, a homotypic adhesion molecule, is expressed by thymocytes and thymic epithelial cells." in: European journal of immunology, Vol. 26, Issue 2, pp. 401-8, (1996) (PubMed).

Borkowski, Nelson, Farr, Udey: "Expression of gp40, the murine homologue of human epithelial cell adhesion molecule (Ep-CAM), by murine dendritic cells." in: European journal of immunology, Vol. 26, Issue 1, pp. 110-4, (1996) (PubMed).

Bergsagel, Victor-Kobrin, Timblin, Trepel, Kuehl: "A murine cDNA encodes a pan-epithelial glycoprotein that is also expressed on plasma cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 2, pp. 590-6, (1992) (PubMed).

Farr, Nelson, Truex, Hosier: "Epithelial heterogeneity in the murine thymus: a cell surface glycoprotein expressed by subcapsular and medullary epithelium." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 39, Issue 5, pp. 645-53, (1991) (PubMed).

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: CD326 (EPCAM Products)

Synonyms: DIAR5 antibody, EGP-2 antibody, EGP314 antibody, EGP40 antibody, ESA antibody, HNPCC8 antibody, KS1/4 antibody, KSA antibody, M4S1 antibody, MIC18 antibody, MK-1 antibody, TACSTD1 antibody, TROP1 antibody, ECS-1 antibody, ECS1 antibody, ESA1 antibody, M17S1 antibody, EGP antibody, Ep-CAM antibody, sb:cb6 antibody, tacstd antibody, wu:fj17g02 antibody, zgc:110304 antibody, zgc:77119 antibody, tacstd1 antibody, MGC80540 antibody, EPCAM antibody, egp antibody, ksa antibody, m4s1 antibody, mk-1 antibody, cd326 antibody, egp40 antibody, mic18 antibody, trop1 antibody, ep-cam antibody, hegp-2 antibody, co17-1a antibody, ga733-2 antibody, CD326 antibody, Egp314 antibody, EpCAM1 antibody, GA733-2 antibody, Ly74 antibody, Tacsd1 antibody, Tacstd1 antibody, gp40 antibody, epithelial cell adhesion molecule antibody, flotillin 2 antibody, epithelial cell adhesion molecule S homeolog antibody, EPCAM antibody, FLOT2 antibody, epcam antibody, epcam.S antibody, Epcam antibody

Background: Synonyms: Ep-CAM