CD3 epsilon antibody (Biotin)

Catalog No. ABIN2689218

There are 4+ publications for this product available. The Golden Syrian Hamster Monoclonal anti-CD3 epsilon antibody is suitable to detect CD3 epsilon in samples from Mouse. It has been validated for FACS.

Quick Overview for CD3 epsilon antibody (Biotin) (ABIN2689218)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Golden Syrian Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 500A2

Product Details anti-CD3 epsilon Antibody (Biotin)

Characteristics: This antibody has been reported to react with the 25- kDa ε chain of the T-cell receptor-associated CD3 complex expressed on thymocytes, mature T lymphocytes, and NK-T cells on many mouse stains. Plate-bound and soluble hamster anti-mouse CD3e (clone 500A2) antibody has been reported to activate unprimed T cells in vitro. Activation of a mouse T-cell clone by soluble hamster anti-mouse CD3e (clone 500A2) can be blocked by Fab fragments of anti-CD4 antibody GK1.5, suggesting that the hamster anti-mouse CD3e (clone 500A2) antibody may bind an epitope of CD3e close to a site at which CD4 associates with the T-cell receptor. This hamster mAb to a mouse leukocyte antigen has been reported not to cross-react with rat leukocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. CD3e expression in spleen and thymus. BALB/c splenocytes were simultaneously stained with PE-conjugated anti-mouse CD4 mAb RM4-5 (Cat. No. 553048/553049, Panels A and C), PE-conjugated anti-mouse CD8a mAb 53-6.7 (Cat. No. 553032/553033, left panels), and biotinylated mAb 500A2 (bottom left panel), followed by Avidin-FITC (Cat. No. 554057, left panels). BALB/c thymocytes were stained with biotinylated 500A2 (bottom right panel) followed by Avidin-FITC (right panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Hamster Anti-Mouse CD3e - Biotin - Clone 500A2 - Isotype Syrian Hamster IgG2, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse T-cell receptor

Isotype: IgG2 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD3 epsilon antibody (Biotin) (ABIN2689218)

Ortaldo, Winkler-Pickett, Mason, Mason: "The Ly-49 family: regulation of cytotoxicity and cytokine production in murine CD3+ cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 3, pp. 1158-65, (1998) (PubMed).

Kubo, Born, Kappler, Marrack, Pigeon: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, (1989) (PubMed).

Portoles, Rojo, Golby, Bonneville, Gromkowski, Greenbaum, Janeway, Murphy, Bottomly: "Monoclonal antibodies to murine CD3 epsilon define distinct epitopes, one of which may interact with CD4 during T cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 12, pp. 4169-75, (1989) (PubMed).

Havran, Poenie, Kimura, Tsien, Weiss, Allison: "Expression and function of the CD3-antigen receptor on murine CD4+8+ thymocytes." in: Nature, Vol. 330, Issue 6144, pp. 170-3, (1987) (PubMed).

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: Synonyms: CD3ε chain

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway