CD44 Standard antibody

Catalog No. ABIN2689266

Product Details anti-CD44 Standard Antibody

Target: CD44 Standard (CD44s)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD44 Standard antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Brand: BD Pharmingen™

Characteristics: The OX-49 antibody reacts with the glycoprotein CD44H (also known as CD44s) expressed on most leukocytes, except for a subset of B lymphocytes, and at greatly increased levels on T- and B-cell blasts. The epitope recognized by OX-49 antibody has been mapped to a region on both the standard, CD44s, and the splice variant, CD44v, isoforms of CD44. However, recent reports indicate that OX-49 antibody cannot detect the CD44v isoform, possibly due to conformational changes in the epitope. CD44 is a cell adhesion receptor, and its ligand, hyaluronate, is a common component of extracellular matrices.

BD Pharmingen™ Purified Mouse Anti-Rat CD44H - Purified - Clone OX-49 - Isotype Mouse IgG2a, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat T blasts from mixed lymphocyte reactions

Clone: OX

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD44 Standard antibody (ABIN2689266)

Noonan, Stevens, Tammi, Tammi, Hernandez, Midura: "Spatial distribution of CD44 and hyaluronan in the proximal tibia of the growing rat." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 14, Issue 4, pp. 573-81, (1996) (PubMed).

Stevens, Noonan, Bosch, Rapp, Martin, Kurriger, Maynard, Daniels, Solursh, Tammi, Tammi, Midura: "CD44 in growing normal and neoplastic rat cartilage." in: Annals of the New York Academy of Sciences, Vol. 785, pp. 333-6, (1996) (PubMed).

Zheng, Katoh, He, Oritani, Miyake, Lesley, Hyman, Hamik, Parkhouse, Farr, Kincade: "Monoclonal antibodies to CD44 and their influence on hyaluronan recognition." in: The Journal of cell biology, Vol. 130, Issue 2, pp. 485-95, (1995) (PubMed).

Westermann, Nagahori, Walter, Heerwagen, Miyasaka, Pabst et al.: "B and T lymphocyte subsets enter peripheral lymph nodes and Peyer's patches without preference in vivo: no correlation occurs between their localization in different types of high endothelial venules ..." in: European journal of immunology, Vol. 24, Issue 10, pp. 2312-6, (1994) (PubMed).

Lesley, Hyman, Kincade: "CD44 and its interaction with extracellular matrix." in: Advances in immunology, Vol. 54, Issue 4, pp. 271-335, (1993) (PubMed).

Arch, Wirth, Hofmann, Ponta, Matzku, Herrlich, Zöller: "Participation in normal immune responses of a metastasis-inducing splice variant of CD44." in: Science (New York, N.Y.), Vol. 257, Issue 5070, pp. 682-5, (1992) (PubMed).

Paterson, Jefferies, Green, Brandon, Corthesy, Puklavec, Williams: "Antigens of activated rat T lymphocytes including a molecule of 50,000 Mr detected only on CD4 positive T blasts." in: Molecular immunology, Vol. 24, Issue 12, pp. 1281-90, (1988) (PubMed).

Target Details for CD44 Standard

Target: CD44 Standard (CD44s)

Alternative Name: CD44H (CD44s Products)

Background: Synonyms: Pgp-1, H-CAM, CD44s