CD45.1 antibody (Biotin)

Catalog No. ABIN2689277

This Mouse Monoclonal antibody specifically detects CD45.1 in FACS and IHC (fro). It exhibits reactivity toward Mouse. It has been mentioned in 6+ publications

Quick Overview for CD45.1 antibody (Biotin) (ABIN2689277)

Target: CD45.1 (CD45 (CD45.1 Allogen))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45.1 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: A20

Product Details anti-CD45.1 Antibody (Biotin)

Characteristics: The A20 antibody reacts with CD45 (Leukocyte Common Antigen) on all leukocytes of mouse strains expressing the CD45.1 alloantigen (eg, RIII, SJL/J, STS/A, DA). This alloantigen was originally named Ly-5.2, and this was the designation at the time that the antibody was characterized. The designation was later changed from Ly-5.2 to Ly-5.1 to conform with the convention that the .2 alloantigen designations be assigned to the C57BL/6 strain. mAb A20 has been reported not to react with leukocytes of most other mouse strains, which express the CD45.2 alloantigen. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. The A20 antibody has been reported to inhibit some responses of B cells, from mice expressing the CD45.1 alloantigen, to antigens and LPS. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Differential expression of CD45.1 in RIII and C57BL/6 spleen. Single-cell suspensions of splenocytes from RIII (left panel) and C57BL/6N (right panel) mice were stained with biotin-conjugated A20 mAb followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-Mouse CD45.1 - Biotin - Clone A20 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: SJL mouse thymocytes and splenocytes

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD45.1 antibody (Biotin) (ABIN2689277)

Suzuki, Oida, Hamada, Hitotsumatsu, Watanabe, Hibi, Yamamoto, Kubota, Kaminogawa, Ishikawa: "Gut cryptopatches: direct evidence of extrathymic anatomical sites for intestinal T lymphopoiesis." in: Immunity, Vol. 13, Issue 5, pp. 691-702, (2001) (PubMed).

Johnson, Greenbaum, Bottomly, Trowbridge: "Identification of the alternatively spliced exons of murine CD45 (T200) required for reactivity with B220 and other T200-restricted antibodies." in: The Journal of experimental medicine, Vol. 169, Issue 3, pp. 1179-84, (1989) (PubMed).

Morse, Shen, Hämmerling: "Genetic nomenclature for loci controlling mouse lymphocyte antigens." in: Immunogenetics, Vol. 25, Issue 2, pp. 71-8, (1987) (PubMed).

Shen, Tung, Boyse: "Further definition of the Ly-5 system." in: Immunogenetics, Vol. 24, Issue 3, pp. 146-9, (1986) (PubMed).

Yakura, Kawabata, Shen, Katagiri: "Selective inhibition of lipopolysaccharide-induced polyclonal IgG response by monoclonal Ly-5 antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 136, Issue 8, pp. 2729-33, (1986) (PubMed).

Yakura, Shen, Bourcet, Boyse: "On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2." in: The Journal of experimental medicine, Vol. 157, Issue 4, pp. 1077-88, (1983) (PubMed).

Target Details for CD45.1

Target: CD45.1 (CD45 (CD45.1 Allogen))

Alternative Name: CD45.1