ITGA2 antibody

Catalog No. ABIN2689302

Product Details anti-ITGA2 Antibody

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This ITGA2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Brand: BD Pharmingen™

Characteristics: The HMα2 antibody reacts with integrin α2 chain (CD49b), the 150- kDa transmembrane glycoprotein that non-covalently associates with the integrin β1 subunit (CD29) to form the integrin α2β1 complex known as VLA-2. VLA-2, a receptor for collagen and laminin, is expressed on some splenic CD4+ T lymphocytes and NK-T cells, intestinal intraepithelial and lamina propria lymphocytes, splenic NK cells, epithelial cells, and platelets, but it is not on thymocytes or Peyer's-patch or lymphnode lymphocytes. The expression of VLA-2 is upregulated on lymphocytes in response to mitogens. The HMα2 antibody has been reported to partially block the interaction of T-cell blasts, but not NK cells, with collagen. Purified HMα2 mAb blocks the staining of splenic NK cells by the anti-CD49b/Pan-NK Cells mAb DX5 (Cat. No. 553858, for the PE conjugate). Therefore, mAb HMα2 may be used like the DX5 mAb for identification of NK cells. Two-color analysis of CD49b expression on spleen leukocytes. C57BL/6 splenocytes were simultaneously stained with FITC-conjugated mAb M1/70 (anti-mouse CD11b, Cat. No. 557396/553310, both panels) and either purified hamster IgG1, κ isotype control mAb A19-3 (Cat. No. 553969, left panel) or purified HMα2 mAb (right panel), followed by PE-conjugated anti-hamster IgG mAb cocktail (Cat. No. 554056, both panels). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.

BD Pharmingen™ Purified Hamster Anti-Mouse CD49b - Purified - Clone HMα2 - Isotype Armenian Hamster IgG1, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse colon carcinoma cell line Colon26

Clone: HMalpha2

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ITGA2 antibody (ABIN2689302)

Arase, Saito, Phillips, Lanier: "Cutting edge: the mouse NK cell-associated antigen recognized by DX5 monoclonal antibody is CD49b (alpha 2 integrin, very late antigen-2)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1141-4, (2001) (PubMed).

Chen, Paul: "A population of CD62Llow Nk1.1- CD4+ T cells that resembles NK1.1+ CD4+ T cells." in: European journal of immunology, Vol. 28, Issue 10, pp. 3172-82, (1998) (PubMed).

Tanaka, Ohtsuka, Yagita, Shiratori, Omata, Okumura: "Involvement of alpha 1 and alpha 4 integrins in gut mucosal injury of graft-versus-host disease." in: International immunology, Vol. 7, Issue 8, pp. 1183-9, (1996) (PubMed).

Noto, Kato, Okumura, Yagita: "Identification and functional characterization of mouse CD29 with a mAb." in: International immunology, Vol. 7, Issue 5, pp. 835-42, (1995) (PubMed).

Miyake, Sakurai, Okumura, Yagita: "Identification of collagen and laminin receptor integrins on murine T lymphocytes." in: European journal of immunology, Vol. 24, Issue 9, pp. 2000-5, (1994) (PubMed).

Target Details for ITGA2

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Alternative Name: CD49b (ITGA2 Products)

Background: Synonyms: Integrin α2 chain

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex