CD5 antibody

Catalog No. ABIN2689320

There are 18+ publications for this product available. The Rat Monoclonal anti-CD5 antibody is suitable to detect CD5 in samples from Mouse. It has been validated for FACS, IP, ELISA, IHC (f), IHC (fro), CyTox and BR.

Quick Overview for CD5 antibody (ABIN2689320)

Target: CD5

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytotoxicity Test (CyTox), Blocking Reagent (BR)

Clone: 53

Product Details anti-CD5 Antibody

Characteristics: The 53-7.3 clone has been reported to react with a monomorphic epitope of CD5, a member of the scavenger receptor cysteine-rich protein superfamily and the major ligand of CD72, found on thymocytes, T lymphocytes, thymic NK-T cells, and a subset of B lymphocytes, but not on NK cells or splenic NK-T cells. The level of surface CD5 expression is developmentally regulated in the thymus, starting with low levels on CD4-CD8- thymocytes and increasing as they mature to CD4+CD8+ then CD4+CD8- or CD4-CD8+ thymocytes. Relatively high levels are maintained on peripheral T lymphocytes. The level of CD5 antigen detected on T helper cells has been reported to be somewhat higher than that on T cytotoxic/suppressor and B cells. Few, if any, intestinal intraepithelial lymphocytes bearing the γδ T-cell receptor express CD5. Phenotypic, anatomical, functional, developmental, and pathogenic characteristics of peripheral CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. The frequency of these CD5+ B cells has been reported to show strain-dependent variation. An additional population of CD5+ B lymphocytes resides in the thymus, where it matures from intrathymic B-cell progenitors. It has been proposed that CD5 is a costimulatory molecule which mediates interactions of cells in the immune system and negatively regulates signal transduction mediated by the T-cell receptor and B-cell receptor. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse CD5 - Purified - Clone 53-7.3 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Thymus / Spleen

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD5 antibody (ABIN2689320)

Lanier: "Natural killer cell receptor signaling." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 308-14, (2003) (PubMed).

Azzam, Grinberg, Lui, Shen, Shores, Love: "CD5 expression is developmentally regulated by T cell receptor (TCR) signals and TCR avidity." in: The Journal of experimental medicine, Vol. 188, Issue 12, pp. 2301-11, (1999) (PubMed).

Bikah, Lynd, Aruffo, Ledbetter, Bondada: "A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5." in: International immunology, Vol. 10, Issue 8, pp. 1185-96, (1998) (PubMed).

Yashiro, Tai, Toyo-oka, Park, Abe, Hamaoka, Kobayashi, Neben, Fujiwara: "A fundamental difference in the capacity to induce proliferation of naive T cells between CD28 and other co-stimulatory molecules." in: European journal of immunology, Vol. 28, Issue 3, pp. 926-35, (1998) (PubMed).

Bendelac, Rivera, Park, Roark: "Mouse CD1-specific NK1 T cells: development, specificity, and function." in: Annual review of immunology, Vol. 15, pp. 535-62, (1997) (PubMed).

Bikah, Carey, Ciallella, Tarakhovsky, Bondada: "CD5-mediated negative regulation of antigen receptor-induced growth signals in B-1 B cells." in: Science (New York, N.Y.), Vol. 274, Issue 5294, pp. 1906-9, (1997) (PubMed).

Masuda, Makino, Cui, Ito, Tokuhisa, Takahama, Koseki, Tsuchida, Koike, Moriya, Amano, Taniguchi: "Phenotypes and invariant alpha beta TCR expression of peripheral V alpha 14+ NK T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 5, pp. 2076-82, (1997) (PubMed).

Mori, Inaba, Sugihara, Taketani, Doi, Fukuba, Yamamoto, Adachi, Inaba, Fukuhara, Ikehara: "Presence of B cell progenitors in the thymus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 9, pp. 4193-9, (1997) (PubMed).

Cibotti, Punt, Dash, Sharrow, Singer: "Surface molecules that drive T cell development in vitro in the absence of thymic epithelium and in the absence of lineage-specific signals." in: Immunity, Vol. 6, Issue 3, pp. 245-55, (1997) (PubMed).

Tarakhovsky, Kanner, Hombach, Ledbetter, Müller, Killeen, Rajewsky: "A role for CD5 in TCR-mediated signal transduction and thymocyte selection." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 535-7, (1995) (PubMed).

Kantor, Herzenberg: "Origin of murine B cell lineages." in: Annual review of immunology, Vol. 11, pp. 501-38, (1993) (PubMed).

Luo, Van de Velde, von Hoegen, Parnes, Thielemans: "Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 6, pp. 1630-4, (1992) (PubMed).

Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, (1991) (PubMed).

Hayakawa, Hardy: "Normal, autoimmune, and malignant CD5+ B cells: the Ly-1 B lineage?" in: Annual review of immunology, Vol. 6, pp. 197-218, (1988) (PubMed).

Hayakawa, Hardy, Parks, Herzenberg: "The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice." in: The Journal of experimental medicine, Vol. 157, Issue 1, pp. 202-18, (1983) (PubMed).

van Ewijk, van Soest, van den Engh: "Fluorescence analysis and anatomic distribution of mouse T lymphocyte subsets defined by monoclonal antibodies to the antigens Thy-1, Lyt-1, Lyt-2, and T-200." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2594-604, (1982) (PubMed).

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, (1980) (PubMed).

Ledbetter, Rouse, Micklem, Herzenberg: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, (1980) (PubMed).

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: Synonyms: Ly-1