ICAM1 antibody

Catalog No. ABIN2689331

This Mouse Monoclonal antibody specifically detects ICAM1 in FACS, IP, IHC (fro), IHC (f) and IHC (zinc). It exhibits reactivity toward Rat and has been mentioned in 13+ publications.

Quick Overview for ICAM1 antibody (ABIN2689331)

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ICAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: 1A29

Product Details anti-ICAM1 Antibody

Brand: BD Pharmingen™

Characteristics: The 1A29 antibody reacts with ICAM-1 (CD54), an 85 kDa surface glycoprotein expressed on vascular endothelium in lymphoid tissues, thymic stromal cells, peripheral blood monocytes, peritoneal macrophages and mast cells, dendritic cells, and weakly on peripheral lymphocytes and thymocytes. ICAM-1 is a ligand for LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). Its expression is upregulated on activated lymphocytes and endothelial cells. 1A29 mAb inhibits PMA-induced aggregation of PHA-stimulated splenic blasts, as well as the adhesion of mitogen-stimulated blasts to HEV-derived endothelial cells and purified ICAM-1. It has also been reported that 1A29 antibody can inhibit leukocyte infiltration in in vivo systems, blocks induction of experimental allergic encephalomyelitis, and reduces NK-cell adhesion to tumor cells. The effects of in vivo administration of the 1A29 monoclonal antibody have been thoroughly investigated. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Rat CD54 - Purified - Clone 1A29 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat HEV-Derived Cell Line Ax

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-ICAM1 antibody (ABIN2689331)

Machelska, Mousa, Brack, Schopohl, Rittner, Schafer, Stein: "Opioid control of inflammatory pain regulated by intercellular adhesion molecule-1." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, Issue 13, pp. 5588-96, (2002) (PubMed).

Liu, Zhang, Jenkins, MacPherson: "Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 3, pp. 1146-55, (1998) (PubMed).

Watanabe, Arakawa, Fukuda, Higuchi, Kobayashi: "Role of neutrophils in a rat model of gastric ulcer recurrence caused by interleukin-1 beta." in: The American journal of pathology, Vol. 150, Issue 3, pp. 971-9, (1997) (PubMed).

Chen-Woan, Delaney, Fournier, Wakizaka, Murase, Fung, Starzl, Demetris: "In vitro characterization of rat bone marrow-derived dendritic cells and their precursors." in: Journal of leukocyte biology, Vol. 59, Issue 2, pp. 196-207, (1996) (PubMed).

Willenborg, Staykova, Miyasaka: "Short term treatment with soluble neuroantigen and anti-CD11a (LFA-1) protects rats against autoimmune encephalomyelitis: treatment abrogates autoimmune disease but not autoimmunity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 5, pp. 1973-80, (1996) (PubMed).

Fox, Jewell, Whitacre: "Rat peritoneal mast cells present antigen to a PPD-specific T cell line." in: Cellular immunology, Vol. 158, Issue 1, pp. 253-64, (1994) (PubMed).

Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).

Westermann, Nagahori, Walter, Heerwagen, Miyasaka, Pabst et al.: "B and T lymphocyte subsets enter peripheral lymph nodes and Peyer's patches without preference in vivo: no correlation occurs between their localization in different types of high endothelial venules ..." in: European journal of immunology, Vol. 24, Issue 10, pp. 2312-6, (1994) (PubMed).

Bañuls, Alvarez, Ferrero, Zapata, Ardavin: "Cell-surface marker analysis of rat thymic dendritic cells." in: Immunology, Vol. 79, Issue 2, pp. 298-304, (1993) (PubMed).

Turunen, Ustinov, Renkonen: "Adhesion molecules involved in protein kinase A- and C-dependent lymphocyte adherence to microvascular endothelial cells." in: Scandinavian journal of immunology, Vol. 37, Issue 3, pp. 282-8, (1993) (PubMed).

Yamazaki, Seko, Tamatani, Miyasaka, Yagita, Okumura, Nagai, Yazaki: "Expression of intercellular adhesion molecule-1 in rat heart with ischemia/reperfusion and limitation of infarct size by treatment with antibodies against cell adhesion molecules." in: The American journal of pathology, Vol. 143, Issue 2, pp. 410-8, (1993) (PubMed).

Tamatani, Kotani, Miyasaka: "Characterization of the rat leukocyte integrin, CD11/CD18, by the use of LFA-1 subunit-specific monoclonal antibodies." in: European journal of immunology, Vol. 21, Issue 3, pp. 627-33, (1991) (PubMed).

Xia, Schneeberger, McCarthy, Kradin: "Accessory cells of the lung. II. Ia+ pulmonary dendritic cells display cell surface antigen heterogeneity." in: American journal of respiratory cell and molecular biology, Vol. 5, Issue 3, pp. 276-83, (1991) (PubMed).

Target Details for ICAM1

Target: ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Alternative Name: CD54

Background: Synonyms: ICAM-1

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling