CD73 antibody

Catalog No. ABIN2689392

Product Details anti-CD73 Antibody

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD73 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Brand: BD Pharmingen™

Characteristics: The 5F/B9 antibody reacts with CD73 or Ecto-5'-nucleotidase (5'-NT), a 68- kDa GPI-anchored cell-surface protein with enzymatic and signal transduction activities. 5'-NT catalyzes the dephosphorylation of extracellular nucleoside 5' monophosphates into a form which can enter cells to meet their metabolic needs. It also regulates the concentration of extracellular adenosine, which initiates a variety of physiological responses through the adenosine receptors in many tissues. Membrane-bound and/or intracytoplasmic CD73 is found in a variety of organs and tissues, including liver, kidney, adrenal cortex, spleen, muscle, nervous tissue, and heart. The expression of CD73 on mouse leukocytes has been well characterized. In the rat, CD73 has been detected on subsets of peripheral B and T lymphocytes, NK cells, and granulocytes, as well as bone marrow myeloid cells and some CD4+CD8- thymocytes. The 5F/B9 mAb does not inhibit the enzymatic activity of 5'-NT. Although soluble or plate-bound 5F/B9 mAb by itself does not affect T lymphocyte proliferation, it is an effective co-stimulator with plate-bound anti-rat CD3 mAb G4.18 (Cat. no. 554829). Two-color analysis of the expression of CD73 on rat spleen leukocytes. LEW splenocytes were stained with FITC- conjugated anti-rat CD45RA mAb OX-33 (Cat. no. 554883) and either purified mouse IgG1, κ isotype control mAb MOPC-31C Cat. no. 557273, Left panel) or purified mAb 5F/B9 (Right panel), followed by biotinylated anti-mouse IgG1 mAb A85-1 (Cat. no. 553441), then Streptavidin-APC (Cat. no. 554067). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Rat CD73 - Purified - Clone 5F/B9 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Ecto-5'-nucleotidase purified from rat renal membranes

Clone: 5F-B9

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD73 antibody (ABIN2689392)

Yamashita, Hooker, Jiang, Laurent, Resta, Khare, Coe, Kincade, Thompson: "CD73 expression and fyn-dependent signaling on murine lymphocytes." in: European journal of immunology, Vol. 28, Issue 10, pp. 2981-90, (1998) (PubMed).

Mlodzik, Loffing, Le Hir, Kaissling: "Ecto-5'-nucleotidase is expressed by pericytes and fibroblasts in the rat heart." in: Histochemistry and cell biology, Vol. 103, Issue 3, pp. 227-36, (1995) (PubMed).

Zenker, Rinne, Bankoul, Le Hir, Kaissling: "5'-nucleotidase in spinal meningeal compartments in the rat. An immuno and enzyme histochemical study." in: Histochemistry, Vol. 98, Issue 2, pp. 135-9, (1992) (PubMed).

Zimmermann: "5'-Nucleotidase: molecular structure and functional aspects." in: The Biochemical journal, Vol. 285 ( Pt 2), pp. 345-65, (1992) (PubMed).

Gandhi, Le Hir, Kaissling: "Immunolocalization of ecto-5'-nucleotidase in the kidney by a monoclonal antibody." in: Histochemistry, Vol. 95, Issue 2, pp. 165-74, (1991) (PubMed).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: CD73 (NT5E Products)

Synonyms: CALJA antibody, CD73 antibody, E5NT antibody, NT antibody, NT5 antibody, NTE antibody, eN antibody, eNT antibody, zgc:63784 antibody, NT5E antibody, MGC114869 antibody, PSPTO2277 antibody, 2210401F01Rik antibody, AI447961 antibody, Nt5 antibody, 5'-nucleotidase ecto antibody, 5'-nucleotidase, ecto (CD73) antibody, 5'-nucleotidase antibody, 5' nucleotidase, ecto antibody, NT5E antibody, nt5e antibody, nt5e.S antibody, PSPTO_2277 antibody, CBU_1233 antibody, MCA1322 antibody, Nt5e antibody

Background: Synonyms: Ecto-5'-nucleotidase

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process