CD80 antibody (Biotin)

Catalog No. ABIN2689412

This Mouse Monoclonal antibody specifically detects CD80 in FACS. It exhibits reactivity toward Rat and has been mentioned in 3+ publications.

Quick Overview for CD80 antibody (Biotin) (ABIN2689412)

Target: CD80

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD80 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Clone: 3H5

Product Details anti-CD80 Antibody (Biotin)

Brand: BD Pharmingen™

Characteristics: The 3H5 antibody reacts with CD80 (B7-1), a member of the Ig superfamily of transmembrane proteins. CD80, a ligand for CD28 and CD152 (CTLA-4), is one of the accessory molecules that plays an important role in T cell-B cell costimulatory interactions. CD80 is predominantly expressed on antigen-presenting cells. It can be induced on splenic B cells by in vitro polyclonal activation such as LPS treatment. 3H5 mAb is reported to block the costimulatory function of rat CD80 and to immunoprecipitate CD80 from Lewis-S1 cell lysates. CD80 expression on spleen cells. 72-hour LPS-stimulated LOU splenocytes were simultaneously stained with FITC-conjugated anti-rat CD45RA mAb OX-33 (Cat. no. 554883) and biotinylated mAb 3H5, followed by Streptavidin-PE (Cat. no. 554061). Viable lymphocyte blasts are represented. Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-Rat CD80 - Biotin - Clone 3H5 - Isotype Mouse IgG1, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat HTLV-1-transformed T-cell line Lewis-S1

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD80 antibody (Biotin) (ABIN2689412)

Damoiseaux, Yagita, Okumura, van Breda Vriesman: "Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." in: Journal of leukocyte biology, Vol. 64, Issue 6, pp. 803-9, (1998) (PubMed).

Maeda, Sato, Azuma, Yagita, Okumura: "Characterization of rat CD80 and CD86 by molecular cloning and mAb." in: International immunology, Vol. 9, Issue 7, pp. 993-1000, (1997) (PubMed).

Bluestone: "New perspectives of CD28-B7-mediated T cell costimulation." in: Immunity, Vol. 2, Issue 6, pp. 555-9, (1995) (PubMed).

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background: Synonyms: B7-1

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints