CD90 antibody

Catalog No. ABIN2689445

Product Details anti-CD90 Antibody

Target: CD90 (THY1)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD90 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Brand: BD Pharmingen™

Characteristics: The G7 antibody reacts with the Thy-1.1 and Thy-1.2 alloantigens (CD90.1 and CD90.2, respectively) on thymocytes, peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), and neurons, but not B lymphocytes, of all mouse strains. CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. In addition, there is evidence that CD90 mediates adhesion of thymocytes to thymic stroma. Soluble G7 mAb stimulates murine T-cell proliferation and IL-2 secretion, via signalling through the TCR/CD3 complex. mAb G7 has been reported to promote apoptosis of thymocytes and CTL clones, as determined by morphological and biochemical criteria. The Thy-1-triggered thymocyte death was found to be developmentally regulated and targeted mainly within the CD4+CD8+CD3- and CD4+CD8+CD3lo cultured immature thymocytes. G7 antibody does not react with Thy-1 (CD90) on rat thymocytes.

BD Pharmingen™ Purified Rat Anti-Mouse CD90 - Purified - Clone G7 - Isotype Rat IgG2c, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse T-cell hybridoma C 6/68

Clone: G7

Isotype: IgG2c kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD90 antibody (ABIN2689445)

Hueber, Raposo, Pierres, He: "Thy-1 triggers mouse thymocyte apoptosis through a bcl-2-resistant mechanism." in: The Journal of experimental medicine, Vol. 179, Issue 3, pp. 785-96, (1994) (PubMed).

Ucker, Meyers, Obermiller: "Activation-driven T cell death. II. Quantitative differences alone distinguish stimuli triggering nontransformed T cell proliferation or death." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 5, pp. 1583-92, (1992) (PubMed).

He, Naquet, Caillol, Pierres: "Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 515-8, (1991) (PubMed).

Sugiyama, Cantagrel, Reno, Stafford-Brady, Yeh, Bonventre: "Selection of T cell receptor expression mutants through the functionally linked Ly-6A." in: Cellular immunology, Vol. 130, Issue 2, pp. 271-80, (1990) (PubMed).

Tentori, Pardoll, Zuñiga, Hu-Li, Paul, Bluestone, Kruisbeek: "Proliferation and production of IL-2 and B cell stimulatory factor 1/IL-4 in early fetal thymocytes by activation through Thy-1 and CD3." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 4, pp. 1089-94, (1988) (PubMed).

Kroczek, Gunter, Germain, Shevach: "Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes." in: Nature, Vol. 322, Issue 6075, pp. 181-4, (1986) (PubMed).

Gunter, Malek, Shevach: "T cell-activating properties of an anti-Thy-1 monoclonal antibody. Possible analogy to OKT3/Leu-4." in: The Journal of experimental medicine, Vol. 159, Issue 3, pp. 716-30, (1984) (PubMed).

Williams, Gagnon: "Neuronal cell Thy-1 glycoprotein: homology with immunoglobulin." in: Science (New York, N.Y.), Vol. 216, Issue 4547, pp. 696-703, (1982) (PubMed).

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90 (THY1 Products)

Synonyms: CD90 antibody, CD7 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, Thy-1 cell surface antigen antibody, thymus cell antigen 1, theta antibody, THY1 antibody, Thy1 antibody

Background: Synonyms: Thy-1

Pathways: Cell-Cell Junction Organization