CD90 antibody (Biotin)

Catalog No. ABIN2689449

Product Details anti-CD90 Antibody (Biotin)

Target: CD90 (THY1)

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD90 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The HIS51 antibody reacts with the rat Thy-1 antigen (CD90) expressed by hematopoietic stem cells, early myeloid and erythroid cells, immature B lymphocytes in the bone marrow and peripheral lymphoid organs, thymocytes, recent thymic emigrants (a subset of CD45RC- peripheral T cells), neurons, glomerular mesangial cells, endothelium at inflammatory sites, mast cells, and bone marrow-derived dendritic cells. Rat dendritic epidermal T cells (DEC) are Thy-1-negative. CD90 is a GPI-anchored membrane glycoprotein of the Ig superfamily which is involved in signal transduction. In addition, there is evidence in the mouse that CD90 mediates adhesion of thymocytes to thymic stroma. HIS51 antibody cross-reacts with the mouse Thy-1.1 alloantigen of the AKR/J and PL strains, but not Thy-1.2 found on most strains. In the mouse, CD90 is found on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), hematopoietic stem cells, and neurons, but not B lymphocytes. Comparison of the expression of CD90/CD90.1 by rat and mouse peripheral lymphocytes. Lewis rat splenocytes were simultaneously stained with PE-conjugated anti-rat CD3 G4.18 (Cat. No. 554833) and biotin-conjugated HIS51 monoclonal antibodies (left panel). P/J mouse splenocytes were simultaneously stained with PE-conjugated anti-mouse CD3e 145-2C11 (Cat. No. 553063/553064) and biotin-conjugated HIS51 monoclonal antibodies (right panel). The second-step reagent was Avidin-FITC (Cat. No. 554057, both panels). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-Rat CD90/Mouse CD90.1 - Biotin - Clone HIS51 - Isotype Mouse IgG2a, κ - Reactivity Rat, Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat bone marrow cells

Clone: HIS51

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CD90 antibody (Biotin) (ABIN2689449)

Chen-Woan, Delaney, Fournier, Wakizaka, Murase, Fung, Starzl, Demetris: "In vitro characterization of rat bone marrow-derived dendritic cells and their precursors." in: Journal of leukocyte biology, Vol. 59, Issue 2, pp. 196-207, (1996) (PubMed).

Dráberová, Amoui, Dráber: "Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains." in: Immunology, Vol. 87, Issue 1, pp. 141-8, (1996) (PubMed).

Ishizu, Ishikura, Nakamaru, Takeuchi, Kimura, Koike, Yoshiki: "Thy-1 induced on rat endothelium regulates vascular permeability at sites of inflammation." in: International immunology, Vol. 7, Issue 12, pp. 1939-47, (1996) (PubMed).

Kroese, de Boer, de Boer, Nieuwenhuis, Kantor, Deenen: "Identification and kinetics of two recently bone marrow-derived B cell populations in peripheral lymphoid tissues." in: Cellular immunology, Vol. 162, Issue 2, pp. 185-93, (1995) (PubMed).

Elbe, Kilgus, Hünig, Stingl: "T-cell receptor diversity in dendritic epidermal T cells in the rat." in: The Journal of investigative dermatology, Vol. 102, Issue 1, pp. 74-9, (1994) (PubMed).

Groen, Klatter, van Petersen, Pater, Nieuwenhuis, Kampinga: "Composition of rat CD4+ resting memory T-cell pool is influenced by major histocompatibility complex." in: Transplantation proceedings, Vol. 25, Issue 5, pp. 2782-3, (1993) (PubMed).

Hosseinzadeh, Goldschneider: "Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 5, pp. 1670-9, (1993) (PubMed).

Nakashima, Pu, Hamaguchi, Iwamoto, Rahman, Zhang, Kato, Ohkusu, Katano, Yoshida: "Pathway of signal delivery to murine thymocytes triggered by co-crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 7, pp. 3511-20, (1993) (PubMed).

He, Naquet, Caillol, Pierres: "Thy-1 supports adhesion of mouse thymocytes to thymic epithelial cells through a Ca2(+)-independent mechanism." in: The Journal of experimental medicine, Vol. 173, Issue 2, pp. 515-8, (1991) (PubMed).

Hermans, Opstelten: "In situ visualization of hemopoietic cell subsets and stromal elements in rat and mouse bone marrow by immunostaining of frozen sections." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 39, Issue 12, pp. 1627-34, (1991) (PubMed).

Paul, Rennke, Milford, Carpenter: "Thy-1.1 in glomeruli of rat kidneys." in: Kidney international, Vol. 25, Issue 5, pp. 771-7, (1984) (PubMed).

Campbell, Gagnon, Reid, Williams: "Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region." in: The Biochemical journal, Vol. 195, Issue 1, pp. 15-30, (1982) (PubMed).

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90.1 (THY1 Products)

Synonyms: CD90 antibody, CD7 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, Thy-1 cell surface antigen antibody, thymus cell antigen 1, theta antibody, THY1 antibody, Thy1 antibody

Background: Synonyms: Thy-1/Thy-1.1

Pathways: Cell-Cell Junction Organization