Chondroitin Sulfate antibody

Catalog No. ABIN2689471

Product Details anti-Chondroitin Sulfate Antibody

Target: Chondroitin Sulfate (CS)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Chondroitin Sulfate antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The 9.2.27 monoclonal antibody specifically binds to CSPG4 (chondroitin sulfate proteoglycan 4). CSPG4 is also known as MCSP (melanoma chondroitin sulfate proteoglycan) and NG2 (neural/glial antigen 2). The hybridoma secreting the 9.2.27 antibody was generated using a human melanoma cell extract as the immunogen. Tumor cells display a variety of antigens which have been defined by antibodies. Antibodies that specifically react with tumor-associated antigens are useful in understanding the biology of particular tumor types. Proteoglycans including chondroitin sulfate, keratan sulfate, dermatan sulfate and heparan sulfate are major components of the extracellular matrices of many animal cell types.

BD Pharmingen™ Purified Mouse Anti-Chondroitin Sulfate - Purified - Clone 9.2.27 - Isotype Mouse IgG2a - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Extracts of Human M21 Melanoma Cells

Clone: 9-2-27

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Chondroitin Sulfate antibody (ABIN2689471)

Schrappe, Bumol, Apelgren, Briggs, Koppel, Markowitz, Mueller, Reisfeld: "Long-term growth suppression of human glioma xenografts by chemoimmunoconjugates of 4-desacetylvinblastine-3-carboxyhydrazide and monoclonal antibody 9.2.27." in: Cancer research, Vol. 52, Issue 14, pp. 3838-44, (1992) (PubMed).

Cheresh, Honsik, Staffileno, Jung, Reisfeld: "Disialoganglioside GD3 on human melanoma serves as a relevant target antigen for monoclonal antibody-mediated tumor cytolysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 15, pp. 5155-9, (1985) (PubMed).

Bumol, Walker, Reisfeld: "Biosynthetic studies of proteoglycans in human melanoma cells with a monoclonal antibody to a core glycoprotein of chondroitin sulfate proteoglycans." in: The Journal of biological chemistry, Vol. 259, Issue 20, pp. 12733-41, (1984) (PubMed).

Morgan, Galloway, Reisfeld: "Production and characterization of monoclonal antibody to a melanoma specific glycoprotein." in: Hybridoma, Vol. 1, Issue 1, pp. 27-36, (1984) (PubMed).

Bumol, Reisfeld: "Unique glycoprotein-proteoglycan complex defined by monoclonal antibody on human melanoma cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 4, pp. 1245-9, (1982) (PubMed).

Target Details for Chondroitin Sulfate

Target: Chondroitin Sulfate (CS)

Alternative Name: Chondroitin Sulfate (CS Products)

Background: Synonyms: CSPG4, NG2, Chondroitin sulfate proteoglycan 4, MCSP, MCSPG, MELCSPG, MSK16