Cutaneous Lymphocyte Antigen antibody (Biotin)

Catalog No. ABIN2689480

Cited in 5+ publications. The Biotin-conjugated Rat Monoclonal anti-Cutaneous Lymphocyte Antigen antibody (Clone HECA) (ABIN2689480) specifically detects Cutaneous Lymphocyte Antigen in FACS, IHC (f) and IHC (fro). The antibody is reactive with Human and Mouse samples.

Quick Overview for Cutaneous Lymphocyte Antigen antibody (Biotin) (ABIN2689480)

Target: Cutaneous Lymphocyte Antigen

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HECA

Product Details

Characteristics: Reacts with cutaneous lymphocyte associated antigen (CLA), a carbohydrate domain shared by sialyl Lewis[x] (sLe[x]) and sialyl Lewis[a] (sLe[a]) antigens. It serves as the ligand for selectins including CD62E (ELAM-1) and CD62L (LECAM-1). CLA is expressed on lymphocytes in the skin on high endothelium. In peripheral blood, it is expressed on a small subset of lympocytes and all monocytes and granulocytes. It has been suggested that CLA plays a role in supporting lymphocyte migration to extravascular tissue during inflammation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed by flow cytometry

BD Pharmingen™ Biotin Rat Anti-Human Cutaneous Lymphocyte Antigen - Biotin - Clone HECA-452 - Isotype Rat IgM, κ - Reactivity Hu, Ms - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Borges, Pendl, Eytner, Steegmaier, Zöllner, Vestweber: "The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated." in: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28786-92, (1997) (PubMed).

Picker, Kishimoto, Smith, Warnock, Butcher: "ELAM-1 is an adhesion molecule for skin-homing T cells." in: Nature, Vol. 349, Issue 6312, pp. 796-9, (1991) (PubMed).

Picker, Warnock, Burns, Doerschuk, Berg, Butcher: "The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140." in: Cell, Vol. 66, Issue 5, pp. 921-33, (1991) (PubMed).

Duijvestijn, Horst, Pals, Rouse, Steere, Picker, Meijer, Butcher: "High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452." in: The American journal of pathology, Vol. 130, Issue 1, pp. 147-55, (1988) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Target Details

Target: Cutaneous Lymphocyte Antigen

Background: Synonyms: CLA