CXCR5 antibody (Biotin)

Catalog No. ABIN2689484

Product Details anti-CXCR5 Antibody (Biotin)

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CXCR5 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The monoclonal antibody 2G8 reacts with the mouse CXC chemokine receptor, CXCR5. CXCR5 (a.k.a. BLR1, NLR and MDR15), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, CXCL13/BLC/BCA-1. The expression of CXCR5 has been detected in spleen, lymph nodes, tonsils, brain, bone marrow, T cells, B cells, cerebrum, cerebellum, hippcampus and pituitary. In mouse spleen, CXCR5 was strictly expressed by mature B cells and a small subset of T lymphocytes. The immunogen used to generate 2G8 hybridoma was a recombinant protein containing N-terminal amino acids of mouse CXCR5 (GST-NmBLR1). This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Detection of CXCR5 expression on mouse splenocytes by biotinylated 2G8. Mouse splenocytes were stained with 1.0 μg/test of biotinylated 2G8, Streptavidin-PE (Cat. No. 554061) and anti-mouse CD45R/B220-FITC (Cat. No. 553088). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using biotinylated rat IgG2a (cat. No. 553928) as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control.

BD Pharmingen™ Biotin Rat Anti-Mouse CXCR5 - Biotin - Clone 2G8 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse CXCR5

Clone: 2G8

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-CXCR5 antibody (Biotin) (ABIN2689484)

Förster, Mattis, Kremmer, Wolf, Brem, Lipp: "A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen." in: Cell, Vol. 87, Issue 6, pp. 1037-47, (1997) (PubMed).

Target Details for CXCR5

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Alternative Name: CXCR5 (CXCR5 Products)

Synonyms: BLR1 antibody, CRL1 antibody, CD185 antibody, MDR15 antibody, Blr1 antibody, CXC-R5 antibody, CXCR-5 antibody, Gpcr6 antibody, NLR antibody, C-X-C motif chemokine receptor 5 antibody, chemokine (C-X-C motif) receptor 5 antibody, CXCR5 antibody, Cxcr5 antibody