Disialoganglioside GD3 antibody

Catalog No. ABIN2689491

There are 5+ publications for this product available. The Mouse Monoclonal anti-Disialoganglioside GD3 antibody is suitable to detect Disialoganglioside GD3 in samples from Human. It has been validated for CyTox, IHC (fro), FACS and IF.

Quick Overview for Disialoganglioside GD3 antibody (ABIN2689491)

Target: Disialoganglioside GD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Cytotoxicity Test (CyTox), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MB3-6

Product Details

Characteristics: Gangliosides are sialic-acid bearing glycolipids expressed on the surface of all mammalian cells, and are likely involved in mediating cell-substratum interactions. They are important target antigens for antibody mediated cytolysis of human melanoma and neuroblastoma cells. Human melanoma cells produce gangliosides GD2 and/or GD3 which are present in substratum-attached material, and may play a significant role in the melanoma metastatic phenotype. Ganglioside GD3 is a major surface marker on most human melanoma cells. MB3.6 has been used to localize GD3 in the plasma membrane and in focal adhesion plaques of human melanoma cells.

BD Pharmingen™ Purified Mouse Anti-Human Disialoganglioside GD3 - Purified - Clone MB3.6 - Isotype Mouse IgG3, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: FM9 human melamona cell line

Isotype: IgG3 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Mujoo, Cheresh, Yang, Reisfeld: "Disialoganglioside GD2 on human neuroblastoma cells: target antigen for monoclonal antibody-mediated cytolysis and suppression of tumor growth." in: Cancer research, Vol. 47, Issue 4, pp. 1098-104, (1987) (PubMed).

Cheresh, Pierschbacher, Herzig, Mujoo: "Disialogangliosides GD2 and GD3 are involved in the attachment of human melanoma and neuroblastoma cells to extracellular matrix proteins." in: The Journal of cell biology, Vol. 102, Issue 3, pp. 688-96, (1986) (PubMed).

Cheresh, Honsik, Staffileno, Jung, Reisfeld: "Disialoganglioside GD3 on human melanoma serves as a relevant target antigen for monoclonal antibody-mediated tumor cytolysis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 15, pp. 5155-9, (1985) (PubMed).

Hakomori: "Tumor-associated carbohydrate antigens." in: Annual review of immunology, Vol. 2, pp. 103-26, (1985) (PubMed).

Cheresh, Harper, Schulz, Reisfeld: "Localization of the gangliosides GD2 and GD3 in adhesion plaques and on the surface of human melanoma cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 18, pp. 5767-71, (1984) (PubMed).

Target Details

Target: Disialoganglioside GD3