CD248 antibody

Catalog No. ABIN2689496

Product Details anti-CD248 Antibody

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD248 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Brand: BD Pharmingen™

Characteristics: The B1/35 monoclonal antibody specifically binds to CD248 which is also known as Endosialin, Tumor endothelial marker 1 (TEM1), and CD164 sialomucin-like 1 (CD164L1). CD248 is a heavily glycosylated, single-pass type I transmembrane protein. CD248 belongs to the Group XIV C-Type lectin family that includes CD93 and CD141/thrombomodulin. It is expressed on pericytes and stromal fibroblasts. Although the exact functions of CD248 remain to be determined, its expression is associated with angiogenesis and lymphoid tissue organization during development, as well as, with postnatal inflammation and tumor development and growth. TOP ROW: Immunohistochemical analysis of Endosialin (CD248) in human tonsil. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections of human tomsil were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, Left Image) or Purified Mouse Anti-Human Endosialin (CD248) (Right Image). A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin HRP (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. As shown in the right image, the CD248-specific antibody primarily stained stromal fibroblasts. Original magnification: 40x. BOTTOM LEFT: Flow cytometric analysis of human Endosialin (CD248) on human palatal mesenchyme cells. HEPM cells (ATTC, CRL-1486) were surface stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121, dotted histogram) or Purified Mouse Anti-Human Endosialin (CD248) (solid line histogram). The second-step reagent was PE Goat Anti-Mouse Ig (Multiple Adsorption, Cat# 550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD FACSCanto™ II Flow Cytometer System. BOTTOM RIGHT: Western blot analysis of human CD248 in human palatal mesenchyme cells. Lysates of HEPM cells (ATTC, CRL-1486) were prepared for electrophoresis (SDS-PAGE) in a 2D Tris-Glycine polyacrylamide gel. The proteins were transferred to PVDF membranes and then probed with 1 (lane 1), 0.5 (lane 2), and 0.25 (lane 3) μg/mL of Purified Mouse Anti-Human Endosialin (CD248). Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat No 554002).

BD Pharmingen™ Purified Mouse Anti-Human Endosialin (CD248) - Purified - Clone B1/35 - Isotype Mouse IgG1, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Diploid human fibroblasts

Clone: B1-35

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CD248 antibody (ABIN2689496)

MacFadyen, Haworth, Roberston, Hardie, Webster, Morris, Panico, Sutton-Smith, Dell, van der Geer, Wienke, Buckley, Isacke: "Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium." in: FEBS letters, Vol. 579, Issue 12, pp. 2569-75, (2005) (PubMed).

Christian, Ahorn, Koehler, Eisenhaber, Rodi, Garin-Chesa, Park, Rettig, Lenter: "Molecular cloning and characterization of endosialin, a C-type lectin-like cell surface receptor of tumor endothelium." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7408-14, (2001) (PubMed).

St Croix, Rago, Velculescu, Traverso, Romans, Montgomery, Lal, Riggins, Lengauer, Vogelstein, Kinzler: "Genes expressed in human tumor endothelium." in: Science (New York, N.Y.), Vol. 289, Issue 5482, pp. 1197-202, (2000) (PubMed).

Rettig, Garin-Chesa, Healey, Su, Jaffe, Old: "Identification of endosialin, a cell surface glycoprotein of vascular endothelial cells in human cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 22, pp. 10832-6, (1992) (PubMed).

Target Details for CD248

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Alternative Name: Endosialin CD248 (CD248 Products)

Synonyms: CD164L1 antibody, TEM1 antibody, 2610111G01Rik antibody, AI842296 antibody, Cd164l1 antibody, Tem1 antibody, fb96b12 antibody, wu:fb96b12 antibody, zgc:162651 antibody, CD248 molecule antibody, CD248 antigen, endosialin antibody, CD248 molecule, endosialin a antibody, CD248 antibody, Cd248 antibody, cd248a antibody

Background: Synonyms: Endosialin, TEM1, CD164L1

Molecular Weight: ~82 kDa