FOXP1 antibody

Catalog No. ABIN2689508

Product Details anti-FOXP1 Antibody

Target: FOXP1 (Forkhead Box P1 (FOXP1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS)

Brand: BD Pharmingen™

Characteristics: The JC12 monoclonal antibody specifically binds to Forkhead box protein P1 (FoxP1). FoxP1 belongs to subfamily P of the forkhead box (FOX) winged-helix transcription factor family. It plays a variety of roles such as regulating the formation of lymphoid, lung, brain and heart tissues. FoxP1 is critical in regulating early B cell development and is also required for maintenance of naive T cell quiescence, and monocyte differentiation and macrophage function. Aberrant expression of FOXP1 has been linked with mucosa-associated lymphoid tissue lymphoma and diffuse-large B cell lymphoma. Panel 1. Western blot analysis of FoxP1 expression in human Ramos cell lysate. Lysate prepared from cells of the human Ramos (Burkitt's lymphoma, ATCC CRL-1596) cell line was electrophoresed (SDS-PAGE), transferred to a polyvinylidene difluoride membrane, and Western blotted using Purified Anti-FoxP1 antibody (Cat. No. 564213, 2 μg/mL, overnight). Human Forkhead box protein P1 was identified as a band whose size ranged from 75-85 kDa. Panel 2. Immunohistochemical staining of acetone-fixed human tonsil for FoxP1. Acetone-fixed, frozen human tonsil sections were stained with either Purified Mouse IgG2a, κ Isotype Control (Cat. No. 554126) or Purified Mouse Anti-FoxP1 antibody. A three-step staining procedure using Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin-HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. The FoxP1-specific antibody primarily stained some lymphocytes and epithelial cells with a nuclear pattern. Original magnification: 20X. Panel 3. Immunohistochemical staining of formalin-fixed human tonsil for FoxP1. Following antigen retrieval with BD Pharmingen™ Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded tonsil sections were stained with either Purified Mouse IgG2a κ Isotype Control or Purified Mouse Anti-Human FoxP1 antibody. A three-step staining procedure was similarly used as before to develop the primary staining reagents. The FoxP1-specific antibody primarily stained some lymphocytes with a nuclear pattern. Original magnification: 40X.

BD Pharmingen™ Purified Mouse Anti-FoxP1 - Purified - Clone JC12 - Isotype Mouse IgG2a, κ - Reactivity Ms, Hu - 50 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: JC12

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-FOXP1 antibody (ABIN2689508)

Sagardoy, Martinez-Ferrandis, Roa, Bunting, Aznar, Elemento, Shaknovich, Fontán, Fresquet, Perez-Roger, Robles, De Smedt, Sagaert, Melnick, Martinez-Climent: "Downregulation of FOXP1 is required during germinal center B-cell function." in: Blood, Vol. 121, Issue 21, pp. 4311-20, (2013) (PubMed).

Katoh, Igarashi, Fukuda, Nakagama, Katoh: "Cancer genetics and genomics of human FOX family genes." in: Cancer letters, Vol. 328, Issue 2, pp. 198-206, (2012) (PubMed).

Feng, Wang, Takata, Day, Willen, Hu: "Transcription factor Foxp1 exerts essential cell-intrinsic regulation of the quiescence of naive T cells." in: Nature immunology, Vol. 12, Issue 6, pp. 544-50, (2011) (PubMed).

Brown, Ashe, Leich, Burek, Barrans, Fenton, Jack, Pulford, Rosenwald, Banham: "Potentially oncogenic B-cell activation-induced smaller isoforms of FOXP1 are highly expressed in the activated B cell-like subtype of DLBCL." in: Blood, Vol. 111, Issue 5, pp. 2816-24, (2008) (PubMed).

Shi, Sakuma, Mooroka, Liscoe, Gao, Croce, Sharma, Kaplan, Greaves, Wang, Simon: "Down-regulation of the forkhead transcription factor Foxp1 is required for monocyte differentiation and macrophage function." in: Blood, Vol. 112, Issue 12, pp. 4699-711, (2008) (PubMed).

Hu, Wang, Borde, Nardone, Maika, Allred, Tucker, Rao: "Foxp1 is an essential transcriptional regulator of B cell development." in: Nature immunology, Vol. 7, Issue 8, pp. 819-26, (2006) (PubMed).

Streubel, Vinatzer, Lamprecht, Raderer, Chott: "T(3;14)(p14.1;q32) involving IGH and FOXP1 is a novel recurrent chromosomal aberration in MALT lymphoma." in: Leukemia, Vol. 19, Issue 4, pp. 652-8, (2005) (PubMed).

Wlodarska, Veyt, De Paepe, Vandenberghe, Nooijen, Theate, Michaux, Sagaert, Marynen, Hagemeijer, De Wolf-Peeters: "FOXP1, a gene highly expressed in a subset of diffuse large B-cell lymphoma, is recurrently targeted by genomic aberrations." in: Leukemia, Vol. 19, Issue 8, pp. 1299-305, (2005) (PubMed).

Wang, Lin, Li, Tucker: "Multiple domains define the expression and regulatory properties of Foxp1 forkhead transcriptional repressors." in: The Journal of biological chemistry, Vol. 278, Issue 27, pp. 24259-68, (2003) (PubMed).

Banham, Beasley, Campo, Fernandez, Fidler, Gatter, Jones, Mason, Prime, Trougouboff, Wood, Cordell: "The FOXP1 winged helix transcription factor is a novel candidate tumor suppressor gene on chromosome 3p." in: Cancer research, Vol. 61, Issue 24, pp. 8820-9, (2001) (PubMed).

Target Details for FOXP1

Target: FOXP1 (Forkhead Box P1 (FOXP1))

Alternative Name: FoxP1 (FOXP1 Products)

Synonyms: 12CC4 antibody, QRF1 antibody, hFKH1B antibody, 3110052D19Rik antibody, 4932443N09Rik antibody, AI461938 antibody, AW494214 antibody, foxp1 antibody, im:7139058 antibody, wu:fc83a06 antibody, FOXP1 antibody, MGC145686 antibody, 12cc4 antibody, fkh1b antibody, hspc215 antibody, qrf1 antibody, xlfoxp1 antibody, im:7146611 antibody, wu:fb97f01 antibody, zgc:154010 antibody, forkhead box P1 antibody, forkhead box P1b antibody, forkhead box P1 S homeolog antibody, forkhead box P1a antibody, FOXP1 antibody, Foxp1 antibody, foxp1b antibody, foxp1 antibody, foxp1.S antibody, foxp1a antibody

Background: Synonyms: Forkhead box protein P1, hFKH1B, HSPC215, QRF1, 12CC4

Molecular Weight: 75-85 kDa

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response