TCR gamma/delta antibody (Biotin)

Catalog No. ABIN2689518

There are 11+ publications for this product available. The Armenian Hamster Monoclonal anti-TCR gamma/delta antibody is suitable to detect TCR gamma/delta in samples from Mouse. It has been validated for FACS and IHC (fro).

Quick Overview for TCR gamma/delta antibody (Biotin) (ABIN2689518)

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This TCR gamma/delta antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: GL3

Product Details anti-TCR gamma/delta Antibody (Biotin)

Characteristics: The GL3 antibody reacts with a common epitope of the δ chain of the T-cell Receptor (TCR) complex on γδ TCR-expressing T lymphocytes and NK-T cells of all mouse strains tested. It does not react with αβ TCR-bearing T cells. In the mouse, cells expressing the γδ TCR are found in the thymus, intestinal epithelium, epidermis, dermis, pulmonsry epithelium, peritoneum, liver, and peripheral lymphoid organs. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of γδ TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotin-conjugated GL3 (right panel) and FITC-conjugated anti-mouse CD3 (Molecular Complex 17A2 (Cat. No. 555274) monoclonal antibodies, followed by Streptavidin-PE (Cat. No. 554061). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Hamster Anti-Mouse γδ T-Cell Receptor - Biotin - Clone GL3 - Isotype Armenian Hamster IgG2, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: C57BL/6 Mouse Intestinal Intraepithelial Lymphocytes

Isotype: IgG2 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-TCR gamma/delta antibody (Biotin) (ABIN2689518)

King, Hyde, Jackson, Novosad, Ellis, Putney, Stovall, Van Winkle, Beaman, Ferrick: "Cutting edge: protective response to pulmonary injury requires gamma delta T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 9, pp. 5033-6, (1999) (PubMed).

Shinohara, Ikarashi, Maruoka, Miyata, Sugimura, Terada, Wakasugi: "Functional and phenotypical characteristics of hepatic NK-like T cells in NK1.1-positive and -negative mouse strains." in: European journal of immunology, Vol. 29, Issue 6, pp. 1871-8, (1999) (PubMed).

Tamaki, Yasaka, Chang, Ohtake, Saitoh, Nakamura, Furue: "Identification and characterization of novel dermal Thy-1 antigen-bearing dendritic cells in murine skin." in: The Journal of investigative dermatology, Vol. 106, Issue 3, pp. 571-5, (1996) (PubMed).

Kaufmann, Blum, Yamamoto: "Crosstalk between alpha/beta T cells and gamma/delta T cells in vivo: activation of alpha/beta T-cell responses after gamma/delta T-cell modulation with the monoclonal antibody GL3." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 20, pp. 9620-4, (1993) (PubMed).

Skeen, Ziegler: "Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection." in: The Journal of experimental medicine, Vol. 178, Issue 3, pp. 971-84, (1993) (PubMed).

Goodman, LeCorre, Lefrancois: "A T-cell receptor gamma delta-specific monoclonal antibody detects a V gamma 5 region polymorphism." in: Immunogenetics, Vol. 35, Issue 1, pp. 65-8, (1992) (PubMed).

Mosley, Klein: "Peripheral engraftment of fetal intestine into athymic mice sponsors T cell development: direct evidence for thymopoietic function of murine small intestine." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1365-73, (1992) (PubMed).

Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, (1991) (PubMed).

MacDonald, Schreyer, Howe, Bron: "Selective expression of CD8 alpha (Ly-2) subunit on activated thymic gamma/delta cells." in: European journal of immunology, Vol. 20, Issue 4, pp. 927-30, (1990) (PubMed).

Tigelaar, Lewis, Bergstresser: "TCR gamma/delta+ dendritic epidermal T cells as constituents of skin-associated lymphoid tissue." in: The Journal of investigative dermatology, Vol. 94, Issue 6 Suppl, pp. 58S-63S, (1990) (PubMed).

Goodman, Lefrancois: "Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function." in: The Journal of experimental medicine, Vol. 170, Issue 5, pp. 1569-81, (1989) (PubMed).

Target Details for TCR gamma/delta

Target: TCR gamma/delta (T-Cell Receptor gamma/delta (TCR gamma/delta))

Alternative Name: Gammadelta T-Cell Receptor