GM-CSF antibody (Biotin)

Catalog No. ABIN2689525

Cited in 4+ publications. The Biotin-conjugated Rat Monoclonal anti-GM-CSF antibody (Clone MP1) (ABIN2689525) specifically detects GM-CSF in ELISA and IP. The antibody is reactive with Mouse samples.

Quick Overview for GM-CSF antibody (Biotin) (ABIN2689525)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is conjugated to Biotin

Application: ELISA, Immunoprecipitation (IP)

Clone: MP1

Product Details anti-GM-CSF Antibody (Biotin)

Characteristics: The MP1-31G6 antibody reacts with mouse granulocyte/macrophage-colony stimulating factor (GM-CSF). The immunogen used to generate this hybridoma was recombinant mouse GM-CSF. This is a neutralizing antibody

BD Pharmingen™ Biotin Rat Anti-Mouse GM-CSF - Biotin - Clone MP1-31G6 - Isotype Rat IgG1 - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant mouse GM-CSF

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-GM-CSF antibody (Biotin) (ABIN2689525)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Nozaki, Abrams, Pearce, Sauder: "Augmentation of granulocyte/macrophage colony-stimulating factor expression by ultraviolet irradiation is mediated by interleukin 1 in Pam 212 keratinocytes." in: The Journal of investigative dermatology, Vol. 97, Issue 1, pp. 10-4, (1991) (PubMed).

Suda, OGarra, MacNeil, Fischer, Bond, Zlotnik: "Identification of a novel thymocyte growth-promoting factor derived from B cell lymphomas." in: Cellular immunology, Vol. 129, Issue 1, pp. 228-40, (1990) (PubMed).

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin