Gp49 Receptor antibody

Catalog No. ABIN2689526

This Armenian Hamster Monoclonal antibody specifically detects Gp49 Receptor in FACS, IP and ELISA. It exhibits reactivity toward Mouse. It has been mentioned in 4+ publications

Quick Overview for Gp49 Receptor antibody (ABIN2689526)

Target: Gp49 Receptor

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA

Clone: H1-1

Product Details

Characteristics: The H1.1 antibody reacts with gp49B, a structural homolog of the human Killer Inhibitory Receptors (KIR), and with the truncated related molecule gp49A. The gp49 receptors are expressed on myeloid cells of adult bone marrow, spleen, and liver and fetal liver. They are not found on resting lymphoid cells, but their expression is upregulated on activated NK cells. gp49B is a member of the Ig superfamily containing two cytoplasmic ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) domains, while gp49A has no ITIM. Although its ligand is unknown, it is established that cross-linking of gp49B can suppress cell activation events.

BD Pharmingen™ Purified Hamster Anti-Mouse gp49 Receptor - Purified - Clone H1.1 - Isotype Armenian Hamster IgG3, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: gp49B-Fc fusion protein composed of extracelluar domain of gp49B

Isotype: IgG3 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

McCormick, Castells, Austen, Katz et al.: "The gp49A gene has extensive sequence conservation with the gp49B gene and provides gp49A protein, a unique member of a large family of activating and inhibitory receptors of the immunoglobulin ..." in: Immunogenetics, Vol. 50, Issue 5-6, pp. 286-94, (2000) (PubMed).

Wang, Chu, Dokun, Yokoyama: "Inducible expression of the gp49B inhibitory receptor on NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 10, pp. 5215-20, (2000) (PubMed).

Wang, Blasioli, Plas, Thomas, Yokoyama: "Specificity of the SH2 domains of SHP-1 in the interaction with the immunoreceptor tyrosine-based inhibitory motif-bearing receptor gp49B." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 3, pp. 1318-23, (1999) (PubMed).

Wang, Mehta, LeBlanc, Yokoyama: "Mouse natural killer cells express gp49B1, a structural homologue of human killer inhibitory receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 1, pp. 13-7, (1997) (PubMed).

Target Details

Target: Gp49 Receptor