Granulocytes antibody

Catalog No. ABIN2689527

There are 4+ publications for this product available. The Mouse Monoclonal anti-Granulocytes antibody is suitable to detect Granulocytes in samples from Rat. It has been validated for FACS, IHC (fro), IHC (f) and IHC (zinc).

Quick Overview for Granulocytes antibody (ABIN2689527)

Target: Granulocytes

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Clone: HIS48

Product Details

Characteristics: The HIS48 antibody reacts with an antigen found on all granulocytes. It is also found on cells of the erythroid lineage at various stages of maturation in bone marrow.

BD Pharmingen™ Purified Mouse Anti-Rat Granulocytes - Purified - Clone HIS48 - Isotype Mouse IgM, κ - Reactivity Rat - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Not reported

Isotype: IgM kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Badger, Sauer, Hoglen, Jolley, Sipes: "The role of inflammatory cells and cytochrome P450 in the potentiation of CCl4-induced liver injury by a single dose of retinol." in: Toxicology and applied pharmacology, Vol. 141, Issue 2, pp. 507-19, (1997) (PubMed).

Kudo, Araki, Matsushima, Sendo: "Inhibition of IL-8-induced W3/25+ (CD4+) T lymphocyte recruitment into subcutaneous tissues of rats by selective depletion of in vivo neutrophils with a monoclonal antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 7, pp. 2196-201, (1991) (PubMed).

van Goor, Fidler, Weening, Grond: "Determinants of focal and segmental glomerulosclerosis in the rat after renal ablation. Evidence for involvement of macrophages and lipids." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 64, Issue 6, pp. 754-65, (1991) (PubMed).

Sekiya, Gotoh, Yamashita, Watanabe, Saitoh, Sendo: "Selective depletion of rat neutrophils by in vivo administration of a monoclonal antibody." in: Journal of leukocyte biology, Vol. 46, Issue 2, pp. 96-102, (1989) (PubMed).

Target Details

Target: Granulocytes