H-2Dd antibody

Catalog No. ABIN2689537

Cited in 9+ publications. The Mouse Monoclonal anti-H-2Dd antibody (Clone 34-5) (ABIN2689537) specifically detects H-2Dd in FACS, IP, CyTox, BR and IAC. The antibody is reactive with Mouse samples.

Quick Overview for H-2Dd antibody (ABIN2689537)

Target: H-2Dd (MHC Class I H-2Dd)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This H-2Dd antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox), Blocking Reagent (BR), Immunoaffinity Chromatography (IAC)

Clone: 34-5

Product Details anti-H-2Dd Antibody

Characteristics: The 34-5-8S antibody recognizes an epitope on the N-terminal domains, α1 and α2, of the H-2Dd. The mAb identifies a conformationally sensitive epitope of H-2Dd associated with β2 microglobulin, it fails to react with free H-2D[d] α chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with other haplotypes (e.g., f, k, p, r) has not been observed. mAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, C+, F+, G2+, or I+ NK cells or transfectants. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Mouse H-2D[d] - Purified - Clone 34-5-8S - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: (C57BL/6 x DBA/2)F1 hybrid mouse splenocytes

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-H-2Dd antibody (ABIN2689537)

Hanke, Takizawa, McMahon, Busch, Pamer, Miller, Altman, Liu, Cado, Lemonnier, Bjorkman, Raulet: "Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors." in: Immunity, Vol. 11, Issue 1, pp. 67-77, (1999) (PubMed).

Chang, Kane: "Evidence for sulfate modification of H-2Dd on N-linked carbohydrate(s): possible involvement in Ly-49A interaction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 9, pp. 4367-74, (1998) (PubMed).

Brennan, Mahon, Mager, Jefferies, Takei: "Recognition of class I major histocompatibility complex molecules by Ly-49: specificities and domain interactions." in: The Journal of experimental medicine, Vol. 183, Issue 4, pp. 1553-9, (1996) (PubMed).

Mason, Ortaldo, Young, Kumar, Bennett, Anderson: "Cloning and functional characteristics of murine large granular lymphocyte-1: a member of the Ly-49 gene family (Ly-49G2)" in: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 293-303, (1995) (PubMed).

Daniels, Karlhofer, Seaman, Yokoyama: "A natural killer cell receptor specific for a major histocompatibility complex class I molecule." in: The Journal of experimental medicine, Vol. 180, Issue 2, pp. 687-92, (1994) (PubMed).

Kane: "Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules." in: The Journal of experimental medicine, Vol. 179, Issue 3, pp. 1011-5, (1994) (PubMed).

Otten, Bikoff, Ribaudo, Kozlowski, Margulies, Germain: "Peptide and beta 2-microglobulin regulation of cell surface MHC class I conformation and expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 12, pp. 3723-32, (1992) (PubMed).

Evans, Margulies, Shykind, Seidman, Ozato: "Exon shuffling: mapping polymorphic determinants on hybrid mouse transplantation antigens." in: Nature, Vol. 300, Issue 5894, pp. 755-7, (1983) (PubMed).

Ozato, Sachs: "Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 126, Issue 1, pp. 317-21, (1981) (PubMed).

Target Details for H-2Dd

Target: H-2Dd (MHC Class I H-2Dd)

Alternative Name: H-2D[d]