HLA-A2 antibody

Catalog No. ABIN2689550

This Mouse Monoclonal antibody specifically detects HLA-A2 in FACS. It exhibits reactivity toward Human.It has been mentioned in 1 publication

Quick Overview for HLA-A2 antibody (ABIN2689550)

Target: HLA-A2

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS)

Clone: BB7-2

Product Details

Characteristics: The monoclonal antibody BB7.2 reacts with a subunit of the human leukocyte antigen-A2 (HLA-A2), a class I molecule of the major histocompatibility complex (MHC). The MHC gene locus encodes a group of highly polymorphic, cell-surface proteins that play a broad role in the immune response to protein antigens. MHC molecules function by binding and presenting small antigenic protein fragments to antigen-specific receptors expressed by T cells (TCR). Human (human leukocyte antigen/HLA) MHC molecules comprise two major classes, MHC class I and class II. Functionally, class I MHC molecules can bind peptides derived from intracellular antigens (eg, viral and some bacterial antigens) that are specifically recognized by CD8+ T cells, and class II MHC molecules recognize antigens derived from pathogens multiplying in intracellular vesicles, and those derived from ingested extracellular bacteria. When presented on the cell surface by the MHC class II molecules, these antigens are recognized by CD4+ T cells. TCR recognize both processed peptides bound to MHC, as well as regions of the MHC molecule itself. CD4 and CD8 accessory molecules strengthen formation of the TCR-MHC complex through their interaction with non-polymorphic regions of the MHC molecule. Profile of peripheral blood lymphocytes analyzed by flow cytometry. Second step staining with Cat. No.55988.

BD Pharmingen™ Purified Mouse Anti-Human HLA-A2 - Purified - Clone BB7.2 - Isotype Mouse IgG2b, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Bjorkman, Saper, Samraoui, Bennett, Strominger, Wiley: "The foreign antigen binding site and T cell recognition regions of class I histocompatibility antigens." in: Nature, Vol. 329, Issue 6139, pp. 512-8, (1987) (PubMed).

Target Details

Target: HLA-A2

Target Type: Influenza Protein