Human Papilloma Virus 16 Capsid Protein (HPV-16 L1) antibody

Catalog No. ABIN2689560

There are 4+ publications for this product available. The Mouse Monoclonal anti-Human Papilloma Virus 16 Capsid Protein antibody is suitable to detect Human Papilloma Virus 16 Capsid Protein in samples from Human. It has been validated for IHC (f) and IHC (fro).

Quick Overview for Human Papilloma Virus 16 Capsid Protein (HPV-16 L1) antibody (ABIN2689560)

Target: Human Papilloma Virus 16 Capsid Protein (HPV-16 L1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: CAMVIR

Product Details

Characteristics: More than 60 different types of human papilloma viruses (HPVs) have been isolated. The human papilloma viruses encode three late proteins, which are produced only in terminally differentiating keratinocytes, two of which (the L1 and L2 proteins) are structural components of the virion. Virus-like particles can be assembled by over-expressing L1 and L2 in vitro, demonstrating that L1 and L2 are necessary and sufficient components of the HPV capsomeres. HPV-16 has been reported to migrate at a reduced molecular weight of ~57 kD by SDS-PAGE. The CAMVIR-1 antibody reacts with the L1 protein of HPV-16 and may also cross-react with other HPV types, such as HPV-33. Immunohistochemistry for HPV type 16. A formalin-fixed, paraffin-embedded section from human cervix infected with HPV type 16 was stained with the Purified Mouse Anti-HPV 16 L1 antibody. Cells expressing the L1 protein of the HPV 16 virus can be identifed by the intense brown labeling (magnification 20X).

BD Pharmingen™ Purified Mouse Anti-HPV-16 L1 - Purified - Clone CAMVIR-1 - Isotype Mouse IgG2a, κ - Reactivity Hu - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Doorbar, Ely, Coleman, Hibma, Davies, Crawford: "Epitope-mapped monoclonal antibodies against the HPV16E1--E4 protein." in: Virology, Vol. 187, Issue 1, pp. 353-9, (1992) (PubMed).

Zhou, Sun, Stenzel, Frazer: "Expression of vaccinia recombinant HPV 16 L1 and L2 ORF proteins in epithelial cells is sufficient for assembly of HPV virion-like particles." in: Virology, Vol. 185, Issue 1, pp. 251-7, (1991) (PubMed).

McLean, Churcher, Meinke, Smith, Higgins, Stanley, Minson: "Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus." in: Journal of clinical pathology, Vol. 43, Issue 6, pp. 488-92, (1990) (PubMed).

de Villiers: "Heterogeneity of the human papillomavirus group." in: Journal of virology, Vol. 63, Issue 11, pp. 4898-903, (1989) (PubMed).

Target Details

Target: Human Papilloma Virus 16 Capsid Protein (HPV-16 L1)

Alternative Name: HPV-16 L1

Target Type: Viral Protein