MHC Class II I-Ab antibody

Catalog No. ABIN2689561

Cited in 4+ publications. The Mouse Monoclonal anti-MHC Class II I-Ab antibody (Clone AF6) (ABIN2689561) specifically detects MHC Class II I-Ab in FACS. The antibody is reactive with Mouse samples.

Quick Overview for MHC Class II I-Ab antibody (ABIN2689561)

Target: MHC Class II I-Ab

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MHC Class II I-Ab antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: AF6

Product Details anti-MHC Class II I-Ab Antibody

Characteristics: The AF6-120.1 antibody reacts with the I-A[b] MHC class II alloantigen. It cross-reacts with cells from mice of the H-2[k] and H-2[u] haplotypes. Reactivity with other haplotypes (e.g., d, f, g7, p, q, r, s) has not been observed. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Mouse I-A[b] - Purified - Clone AF6-120.1 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse C57BL/10J

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-MHC Class II I-Ab antibody (ABIN2689561)

Nabozny, Baisch, Cheng, Cosgrove, Griffiths, Luthra, David: "HLA-DQ8 transgenic mice are highly susceptible to collagen-induced arthritis: a novel model for human polyarthritis." in: The Journal of experimental medicine, Vol. 183, Issue 1, pp. 27-37, (1996) (PubMed).

Cohn, Glimcher, Waldmann, Smith, Ben-Nun, Seidman, Choi: "Identification of functional regions on the I-Ab molecule by site-directed mutagenesis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 747-51, (1986) (PubMed).

Hattori, Buse, Jackson, Glimcher, Dorf, Minami, Makino, Moriwaki, Kuzuya, Imura: "The NOD mouse: recessive diabetogenic gene in the major histocompatibility complex." in: Science (New York, N.Y.), Vol. 231, Issue 4739, pp. 733-5, (1986) (PubMed).

Wall, Lorber, Loken, McClatchey, Fitch: "Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 3, pp. 1056-64, (1983) (PubMed).

Target Details for MHC Class II I-Ab

Target: MHC Class II I-Ab

Alternative Name: I-A[b]