I-AD antibody

Catalog No. ABIN2689568

This Mouse Monoclonal antibody specifically detects I-AD in FACS and BR. It exhibits reactivity toward Mouse. It has been mentioned in 4+ publications

Quick Overview for I-AD antibody (ABIN2689568)

Target: I-AD

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: AMS

Product Details

Characteristics: The AMS-32.1 antibody reacts with the I-A[d] MHC class II alloantigen. It cross-reacts with cells from mice of the H-2[f], H-2[g7], H-2[i], and H-2[v] haplotypes. Reactivity with other haplotypes (e.g., k, p, q, r, s, u) has not been observed.

BD Pharmingen™ Purified Mouse Anti-Mouse I-A[d] - Purified - Clone AMS-32.1 - Isotype Mouse IgG2b, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: BALB/c mouse splenocytes

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Ridgway, Ito, Fassò, Yu, Fathman: "Analysis of the role of variation of major histocompatibility complex class II expression on nonobese diabetic (NOD) peripheral T cell response." in: The Journal of experimental medicine, Vol. 188, Issue 12, pp. 2267-75, (1999) (PubMed).

Hiramine, Nakagawa, Hojo: "Murine nursing thymic epithelial cell lines capable of inducing thymocyte apoptosis express the self-superantigen Mls-1a." in: Cellular immunology, Vol. 160, Issue 1, pp. 157-62, (1995) (PubMed).

Wall, Lorber, Loken, McClatchey, Fitch: "Inhibition of proliferation of MIs- and Ia-reactive cloned T cells by a monoclonal antibody against a determinant shared by I-A and I-E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 3, pp. 1056-64, (1983) (PubMed).

Loken, Stall: "Flow cytometry as an analytical and preparative tool in immunology." in: Journal of immunological methods, Vol. 50, Issue 3, pp. R85-112, (1982) (PubMed).

Target Details

Target: I-AD

Alternative Name: I-A[d]