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I-AD antibody (Biotin)

The Biotin-conjugated Mouse Monoclonal anti-I-AD antibody (Clone AMS) (ABIN2689570) specifically detects I-AD in FACS, IHC (fro) and IHC (p). The antibody is reactive with Mouse samples.
Catalog No. ABIN2689570
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Quick Overview for I-AD antibody (Biotin) (ABIN2689570)

Target

I-AD

Reactivity

Mouse

Host

  • 1
Mouse

Clonality

  • 1
Monoclonal

Conjugate

  • 1
Biotin

Application

Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

AMS
  • Characteristics

    The AMS-32.1 antibody reacts with the I-A[d] MHC class II alloantigen. It cross-reacts with cells from mice of the H-2[f], H-2[g7], H-2[i], and H-2[v] haplotypes. Reactivity with other haplotypes (e.g., k, p, q, r, s, u) has not been observed. Immunohistochemistry of I-A[d] positive cells. Frozen sections of mouse thymus were reacted with the anti-I-A[d] antibody. Cells expressing the MHC class II molecule I-A[d] can be identified by the intense brown labeling of their cell membranes. Magnification 20x.

    BD Pharmingen™ Biotin Mouse Anti-Mouse I-A[d] - Biotin - Clone AMS-32.1 - Isotype Mouse IgG2b, κ - Reactivity Ms - 1 mL

    Purification

    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

    Immunogen

    BALB/c mouse splenocytes

    Isotype

    IgG2b kappa
  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    62.5 μg/mL

    Buffer

    Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

    Storage

    4 °C

    Storage Comment

    Store undiluted at 4°C.
  • Target

    I-AD

    Alternative Name

    I-A[d]
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