MHC II (I-A/I-E) antibody

Catalog No. ABIN2689576

Cited in 7+ publications. The Rat Monoclonal anti-MHC II (I-A/I-E) antibody (Clone M5-114-15-2) (ABIN2689576) specifically detects MHC II (I-A/I-E) in FACS, IP, IF, BR, IHC (fro) and ICS. The antibody is reactive with Mouse samples.

Quick Overview for MHC II (I-A/I-E) antibody (ABIN2689576)

Target: MHC II (I-A/I-E)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MHC II (I-A/I-E) antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS)

Clone: M5-114-15-2

Product Details anti-MHC II (I-A/I-E) Antibody

Characteristics: The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-A[b], I-A[d], I-A[q], I-E[d], and I-E[k] (but not I-A[f], I-A[k], or I-A[s]) MHC class II alloantigens. It also reacts with cells from mice of the H-2[p] and H-2[r] haplotypes, and it is non-reactive with cells from NOD (H-2[g7]) mice. Flow cytometric analysis indicates that the M5/114.15.2 and 2G9 (Cat. No. 553621) monoclonal antibodies have comparable reactivity on cells from mice with I-A[b], I-A[d], I-A[g7], I-A[q], I-E[d], and I-E[k] alloantigens. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Rat Anti-Mouse I-A/I-E - Purified - Clone M5/114.15.2 (also known as M5/114) - Isotype Rat IgG2b, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Activated C57BL/6 Mouse Spleen

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-MHC II (I-A/I-E) antibody (ABIN2689576)

Shi, Kaliyaperumal, Lu, Southwood, Sette, Michaels, Datta: "Promiscuous presentation and recognition of nucleosomal autoepitopes in lupus: role of autoimmune T cell receptor alpha chain." in: The Journal of experimental medicine, Vol. 187, Issue 3, pp. 367-78, (1998) (PubMed).

Guo, Watanabe, Mori, Mori: "Molecular structure and function of CD4 on murine egg plasma membrane." in: Zygote (Cambridge, England), Vol. 3, Issue 1, pp. 65-73, (1995) (PubMed).

Yamashita, Nagata, Tada, Nakayama: "CD69 cell surface expression identifies developing thymocytes which audition for T cell antigen receptor-mediated positive selection." in: International immunology, Vol. 5, Issue 9, pp. 1139-50, (1994) (PubMed).

Nelson, Hosier, Brady, Linsley, Farr: "Medullary thymic epithelium expresses a ligand for CTLA4 in situ and in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2453-61, (1993) (PubMed).

Viville, Neefjes, Lotteau, Dierich, Lemeur, Ploegh, Benoist, Mathis: "Mice lacking the MHC class II-associated invariant chain." in: Cell, Vol. 72, Issue 4, pp. 635-48, (1993) (PubMed).

Hattori, Buse, Jackson, Glimcher, Dorf, Minami, Makino, Moriwaki, Kuzuya, Imura: "The NOD mouse: recessive diabetogenic gene in the major histocompatibility complex." in: Science (New York, N.Y.), Vol. 231, Issue 4739, pp. 733-5, (1986) (PubMed).

Bhattacharya, Dorf, Springer: "A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 127, Issue 6, pp. 2488-95, (1982) (PubMed).

Target Details for MHC II (I-A/I-E)

Target: MHC II (I-A/I-E)

Alternative Name: I-A/I-E