IL-3 antibody

Catalog No. ABIN2689758

The Rat Monoclonal anti-IL-3 antibody has been validated for ELISA, Neut and Inhibition. It is suitable to detect IL-3 in samples from Mouse. There are 5+ publications available.

Quick Overview for IL-3 antibody (ABIN2689758)

Target: IL-3 (Interleukin 3 (IL-3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-3 antibody is un-conjugated

Application: ELISA, Neutralization (Neut), Blocking Antibody (Inhibition)

Clone: MP2

Product Details anti-IL-3 Antibody

Brand: BD Pharmingen™

Characteristics: The MP2-8F8 antibody reacts with mouse interleukin-3 (IL-3). The immunogen used to generate the MP2-8F8 hybridoma was COS-expressed recombinant mouse IL-3. This is a neutralizing antibody. ELISA standard curve for Mouse IL-3.

BD Pharmingen™ Purified Rat Anti-Mouse IL-3 - Purified - Clone MP2-8F8 - Isotype Rat IgG1 - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant mouse IL-3

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-IL-3 antibody (ABIN2689758)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Cockayne, Muchamuel, Grimaldi, Muller-Steffner, Randall, Lund, Murray, Schuber, Howard: "Mice deficient for the ecto-nicotinamide adenine dinucleotide glycohydrolase CD38 exhibit altered humoral immune responses." in: Blood, Vol. 92, Issue 4, pp. 1324-33, (1998) (PubMed).

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Abrams, Pearce: "Development of rat anti-mouse interleukin 3 monoclonal antibodies which neutralize bioactivity in vitro." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 1, pp. 131-7, (1988) (PubMed).

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL-3

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy