LGR5 antibody (N-Term)

Catalog No. ABIN2689804

Product Details anti-LGR5 Antibody

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Binding Specificity: N-Term

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This LGR5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), BioImaging (BI)

Brand: BD Pharmingen™

Cross-Reactivity (Details): Mouse (Lack of Reactivity Confirmed in Development)

Characteristics: Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) is a seven transmembrane-domain receptor that is a target gene for Wnt and marks stem cells in the small intestine, colon, stomach, and hair follicle. Lgr5 was initially identified as a potential stem cell marker due to restricted expression of Lgr5 in the intestinal crypt and labeling of rapidly cycling cells of the colon and intestine. Using both lineage tracing and organoid culture experiments, Lgr5 positive cells are capable of generating all types of the small intestine epithelium hence indicating that Lgr5 marks stem cells of the small intestine and colon. R-spondin growth factors, which are secreted agonists of the Wnt pathway, bind Lgr5. The binding of R-spondins to Lgr5 leads to recruitment of the Frizzled/LRP Wnt receptor complex, which binds to Wnt ligands and leads to downstream Wnt signaling. Lgr5 is up-regulated in colon and ovarian cancers and has been implicated in promotion of tumor growth and metastasis. The 8F2 monoclonal antibody recognizes an epitope in the N-terminal region of Human Lgr5. Western Blot analysis of human LGR5. Lysates from human epithelial kidney cells, HEK293T, that were transiently transfected with empty vector (lane 1) or human LGR5 (lane 2) were probed with Purified Rat anti-human LGR5 monoclonal antibody (

BD Pharmingen™ Purified Rat anti-Human Lgr5 (N-Terminal) - Purified - Clone 8F2 - Isotype Rat IgG2b, κ - Reactivity Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human LGR5 DNA

Clone: 8F2

Isotype: IgG2b kappa

Application Details

Application Notes: Immunohistochemistry-formalin (antigen retrieval required), Immunohistochemistry-frozen (Not Recommended)

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-LGR5 antibody (ABIN2689804)

Yui, Nakamura, Sato, Nemoto, Mizutani, Zheng, Ichinose, Nagaishi, Okamoto, Tsuchiya, Clevers, Watanabe: "Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell." in: Nature medicine, Vol. 18, Issue 4, pp. 618-23, (2012) (PubMed).

Carmon, Gong, Lin, Thomas, Liu: "R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 28, pp. 11452-7, (2011) (PubMed).

Merlos-Suárez, Barriga, Jung, Iglesias, Céspedes, Rossell, Sevillano, Hernando-Momblona, da Silva-Diz, Muñoz, Clevers, Sancho, Mangues, Batlle: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, (2011) (PubMed).

de Lau, Barker, Low, Koo, Li, Teunissen, Kujala, Haegebarth, Peters, van de Wetering, Stange, van Es, Guardavaccaro, Schasfoort, Mohri, Nishimori, Mohammed, Heck, Clevers: "Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling." in: Nature, Vol. 476, Issue 7360, pp. 293-7, (2011) (PubMed).

Barker, Huch, Kujala, van de Wetering, Snippert, van Es, Sato, Stange, Begthel, van den Born, Danenberg, van den Brink, Korving, Abo, Peters, Wright, Poulsom, Clevers: "Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro." in: Cell stem cell, Vol. 6, Issue 1, pp. 25-36, (2010) (PubMed).

Jaks, Barker, Kasper, van Es, Snippert, Clevers, Toftgård: "Lgr5 marks cycling, yet long-lived, hair follicle stem cells." in: Nature genetics, Vol. 40, Issue 11, pp. 1291-9, (2008) (PubMed).

Barker, van Es, Kuipers, Kujala, van den Born, Cozijnsen, Haegebarth, Korving, Begthel, Peters, Clevers: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, (2007) (PubMed).

Target Details for LGR5

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Alternative Name: Lgr5 (LGR5 Products)

Synonyms: lgr5-a antibody, lgr5a antibody, FEX antibody, Gpr49 antibody, GPR49 antibody, GPR67 antibody, GRP49 antibody, HG38 antibody, leucine-rich repeat containing G protein-coupled receptor 5 L homeolog antibody, leucine rich repeat containing G protein coupled receptor 5 antibody, leucine rich repeat containing G protein-coupled receptor 5 antibody, lgr5.L antibody, Lgr5 antibody, LGR5 antibody

Background: Synonyms: GPR49, GPR67, HG38

Molecular Weight: ~110 kDa

Pathways: WNT Signaling