Lgr5 Central LRR antibody (Biotin)

Catalog No. ABIN2689807

Cited in 8+ publications. The Biotin-conjugated Rat Monoclonal anti-Lgr5 Central LRR antibody (Clone 4D11F8) (ABIN2689807) specifically detects Lgr5 Central LRR in BI, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for Lgr5 Central LRR antibody (Biotin) (ABIN2689807)

Target: Lgr5 Central LRR

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: Biotin

Application: BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 4D11F8

Product Details

Characteristics: Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) is a seven transmembrane-domain receptor that is a target gene for Wnt and marks stem cells in the small intestine, colon, stomach, and hair follicle. Lgr5 was initially identified as a potential stem cell marker due to restricted expression of Lgr5 in the intestinal crypt and labeling of rapidly cycling cells of the colon and intestine. Using both lineage tracing and organoid culture experiments, Lgr5 positive cells are capable of generating all types of the small intestine epithelium hence indicating that Lgr5 marks stem cells of the small intestine and colon. R-spondin growth factors, which are secreted agonists of the Wnt pathway, bind Lgr5. The binding of R-spondins to Lgr5 leads to recruitment of the Frizzled/LRP Wnt receptor complex, which binds to Wnt ligands and leads to downstream Wnt signaling. Lgr5 is up-regulated in colon and ovarian cancers and has been implicated in promotion of tumor growth and metastasis. The 4D11F8 monoclonal antibody recognizes an epitope in the center of the leucine-rich repeat (LRR) region of Human Lgr5. Flow cytometric analysis of LGR5 expression on human LGR5-transfected cells. LS 174T colorectal adenocarcinoma cells transfected with human LGR5 (cells from Dr. Hans Clevers, Hubrecht Institute, Left Panel) and parental LS 174T cells, which express low levels of endogenous LGR5 (ATCC CL-188, Right Panel), were harvested using Accutase™ Cell Detachment Solution (Cat. No. 561527). The cells were stained with either Biotin Rat IgG2b, κ Isotype Control (Cat. No. 553987, dashed line histogram) or Biotin Rat anti-Human Lgr5 (Central LRR) antibody (Cat. No. 562904, solid line histogram) at matched concentrations. The cells were then washed and stained with PE Streptavidin (Cat. No. 554061) as the secondary detection reagent. The fluorescence histograms were derived from gated events based on the light scattering characteristics of viable LS 174T cells. Flow cytometry was performed on a BD FACSCanto™ II

BD Pharmingen™ Biotin Rat anti-Human Lgr5 (Central LRR) - Biotin - Clone 4D11F8 (also known as 4D11) - Isotype Rat IgG2b, λ - Reactivity Hu - 50 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human LGR5 DNA

Isotype: IgG2b lambda

Application Details

Application Notes: Flow Cytometry System.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Kemper, Prasetyanti, De Lau, Rodermond, Clevers, Medema: "Monoclonal antibodies against Lgr5 identify human colorectal cancer stem cells." in: Stem cells (Dayton, Ohio), Vol. 30, Issue 11, pp. 2378-86, (2012) (PubMed).

Yui, Nakamura, Sato, Nemoto, Mizutani, Zheng, Ichinose, Nagaishi, Okamoto, Tsuchiya, Clevers, Watanabe: "Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell." in: Nature medicine, Vol. 18, Issue 4, pp. 618-23, (2012) (PubMed).

Carmon, Gong, Lin, Thomas, Liu: "R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 28, pp. 11452-7, (2011) (PubMed).

Merlos-Suárez, Barriga, Jung, Iglesias, Céspedes, Rossell, Sevillano, Hernando-Momblona, da Silva-Diz, Muñoz, Clevers, Sancho, Mangues, Batlle: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, (2011) (PubMed).

de Lau, Barker, Low, Koo, Li, Teunissen, Kujala, Haegebarth, Peters, van de Wetering, Stange, van Es, Guardavaccaro, Schasfoort, Mohri, Nishimori, Mohammed, Heck, Clevers: "Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling." in: Nature, Vol. 476, Issue 7360, pp. 293-7, (2011) (PubMed).

Barker, Huch, Kujala, van de Wetering, Snippert, van Es, Sato, Stange, Begthel, van den Born, Danenberg, van den Brink, Korving, Abo, Peters, Wright, Poulsom, Clevers: "Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro." in: Cell stem cell, Vol. 6, Issue 1, pp. 25-36, (2010) (PubMed).

Jaks, Barker, Kasper, van Es, Snippert, Clevers, Toftgård: "Lgr5 marks cycling, yet long-lived, hair follicle stem cells." in: Nature genetics, Vol. 40, Issue 11, pp. 1291-9, (2008) (PubMed).

Barker, van Es, Kuipers, Kujala, van den Born, Cozijnsen, Haegebarth, Korving, Begthel, Peters, Clevers: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, (2007) (PubMed).

Target Details

Target: Lgr5 Central LRR

Background: Synonyms: GPR49, GPR67, HG38