Lgr5 CRL Region antibody

Catalog No. ABIN2689808

This Rat Monoclonal antibody specifically detects Lgr5 CRL Region in BI, FACS, IF and WB. It exhibits reactivity toward Human. It has been mentioned in 8+ publications

Quick Overview for Lgr5 CRL Region antibody (ABIN2689808)

Target: Lgr5 CRL Region

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Application: BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: 1D9A5

Product Details

Cross-Reactivity (Details): Mouse (Lack of Reactivity Confirmed in Development)

Characteristics: Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) is a seven transmembrane-domain receptor that is a target gene for Wnt and marks stem cells in the small intestine and colon, stomach, and hair follicle. Lgr5 was initially identified as a potential stem cell marker due to restricted expression of Lgr5 in the intestinal crypt and labeling of rapidly cycling cells of the colon and intestine. Using both lineage tracing and organiod culture experiments, Lgr5 positive cells are capable of generating all types of the small intestine epithelium hence indicating that Lgr5 marks stem cells of the small intestine and colon. R-spondin growth factors, which are secreted agonists of the Wnt pathway, bind Lgr5. The binding of R-spondins to Lgr5 leads to recruitment of the Frizzled/LRP Wnt receptor complex, which binds to Wnt ligands and leads to downstream Wnt signaling. Lgr5 is up-regulated in colon and ovarian cancers and has been implicated in promotion of tumor growth and metastasis. The 1D9A5 monoclonal antibody recognizes an epitope in the Cysteine-rich linker (CRL) region of Human Lgr5. Western blot analysis of human Lgr5. Lysate from colorectal adenocarcinoma cells LS 174T transfected with human LGR5 (Cells from Dr. Hans Clevers, Hubrecht Institute) was probed with Purified Rat anti-Human Lgr5 (CRL Region) monoclonal antibody (Cat. No. 562733) at titrations of 1.0 (lane 1), 0.5 (lane 2), and 0.25 μg/mL (lane 3). Lgr5 is identified as a strong band of ~110 kDa. The protein was detected using HRP Goat Anti-Rat Ig (Cat. No. 554017) and a chemiluminescent detection system. Immunofluorescent staining of human Lgr5. Colorectal adenocarcinoma cells LS 174T transfected with human LGR5 (Cells from Dr. Hans Clevers, Hubrecht Institute) were stained with Purified Rat anti-Human Lgr5 (CRL Region) monoclonal antibody (Cat. No. 562733) (pseudo colored green) at 5 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-Rat IgG (Life Technologies). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software.

BD Pharmingen™ Purified Rat anti-Human Lgr5 (CRL Region) - Purified - Clone 1D9A5 (also known as 1D9) - Isotype Rat IgG2b, λ - Reactivity Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human LGR5 DNA

Isotype: IgG2b lambda

Application Details

Application Notes: Immunohistochemistry-frozen, Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Kemper, Prasetyanti, De Lau, Rodermond, Clevers, Medema: "Monoclonal antibodies against Lgr5 identify human colorectal cancer stem cells." in: Stem cells (Dayton, Ohio), Vol. 30, Issue 11, pp. 2378-86, (2012) (PubMed).

Yui, Nakamura, Sato, Nemoto, Mizutani, Zheng, Ichinose, Nagaishi, Okamoto, Tsuchiya, Clevers, Watanabe: "Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell." in: Nature medicine, Vol. 18, Issue 4, pp. 618-23, (2012) (PubMed).

Carmon, Gong, Lin, Thomas, Liu: "R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 28, pp. 11452-7, (2011) (PubMed).

Merlos-Suárez, Barriga, Jung, Iglesias, Céspedes, Rossell, Sevillano, Hernando-Momblona, da Silva-Diz, Muñoz, Clevers, Sancho, Mangues, Batlle: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, (2011) (PubMed).

de Lau, Barker, Low, Koo, Li, Teunissen, Kujala, Haegebarth, Peters, van de Wetering, Stange, van Es, Guardavaccaro, Schasfoort, Mohri, Nishimori, Mohammed, Heck, Clevers: "Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling." in: Nature, Vol. 476, Issue 7360, pp. 293-7, (2011) (PubMed).

Barker, Huch, Kujala, van de Wetering, Snippert, van Es, Sato, Stange, Begthel, van den Born, Danenberg, van den Brink, Korving, Abo, Peters, Wright, Poulsom, Clevers: "Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro." in: Cell stem cell, Vol. 6, Issue 1, pp. 25-36, (2010) (PubMed).

Jaks, Barker, Kasper, van Es, Snippert, Clevers, Toftgård: "Lgr5 marks cycling, yet long-lived, hair follicle stem cells." in: Nature genetics, Vol. 40, Issue 11, pp. 1291-9, (2008) (PubMed).

Barker, van Es, Kuipers, Kujala, van den Born, Cozijnsen, Haegebarth, Korving, Begthel, Peters, Clevers: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, (2007) (PubMed).

Target Details

Target: Lgr5 CRL Region

Background: Synonyms: GPR49, GPR67, HG38

Molecular Weight: ~110 kDa