Monocyte/Granulocyte antibody

Catalog No. ABIN2689848

There are 7+ publications for this product available. The Mouse Monoclonal anti-Monocyte/Granulocyte antibody is suitable to detect Monocyte/Granulocyte in samples from Pig. It has been validated for FACS, IP and IHC (fro).

Quick Overview for Monocyte/Granulocyte antibody (ABIN2689848)

Target: Monocyte/Granulocyte

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Monocyte/Granulocyte antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 74-22

Product Details anti-Monocyte/Granulocyte Antibody

Characteristics: The 74-22-15A (switch variant of 74-22-15) antibody, an isotype class-switch variant of mAb 74-22-15, reacts with a 230- kDa protein1 expressed by most macrophages, peripheral blood monocytes and granulocytes, and few lymphocytes. mAb 74-22-15A does not cross-react with human or bovine cells. This clone was clustered as anti-SWC3a at the First International Swine CD workshop.

BD Pharmingen™ Purified Mouse Anti-Pig Monocyte/Granulocyte - Purified - Clone 74-22-15A - Isotype Mouse IgG2b, κ - Reactivity Pig - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: dd miniature swine thymocytes

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-Monocyte/Granulocyte antibody (ABIN2689848)

Summerfield, Haverson, Thacker, McCullough: "Differentiation of porcine myeloid bone marrow haematopoietic cell populations." in: Veterinary immunology and immunopathology, Vol. 80, Issue 1-2, pp. 121-9, (2001) (PubMed).

McCullough, Schaffner, Natale, Kim, Summerfield: "Phenotype of porcine monocytic cells: modulation of surface molecule expression upon monocyte differentiation into macrophages." in: Veterinary immunology and immunopathology, Vol. 58, Issue 3-4, pp. 265-75, (1998) (PubMed).

Binns, Whyte, Licence, Harrison, Tsang, Haskard, Robinson: "The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 9, pp. 4094-9, (1996) (PubMed).

Yang, Parkhouse: "Phenotypic classification of porcine lymphocyte subpopulations in blood and lymphoid tissues." in: Immunology, Vol. 89, Issue 1, pp. 76-83, (1996) (PubMed).

Blecha, Kielian, McVey, Lunney, Walker, Stokes, Stevens, Kim, Chu, Chen: "Workshop studies on monoclonal antibodies reactive against porcine myeloid cells." in: Veterinary immunology and immunopathology, Vol. 43, Issue 1-3, pp. 269-72, (1995) (PubMed).

Sundt, Arn, Sachs: "Patterns of T cell-accessory cell interaction in the generation of primary alloresponses in the pig." in: Transplantation, Vol. 54, Issue 5, pp. 911-6, (1992) (PubMed).

Pescovitz, Lunney, Sachs: "Preparation and characterization of monoclonal antibodies reactive with porcine PBL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 133, Issue 1, pp. 368-75, (1984) (PubMed).

Target Details for Monocyte/Granulocyte

Target: Monocyte/Granulocyte

Background: Synonyms: SWC3a