NK-1.1/CD161c antibody

Catalog No. ABIN2689857

Product Details anti-NK-1.1/CD161c Antibody

Target: NK-1.1/CD161c (NK-1.1)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NK-1.1/CD161c antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Cytotoxicity Test (CyTox), Blocking Reagent (BR), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: In the mouse, at least three members of the Klrb (Killer cell lectin-like receptor, subfamily b, formerly NKR-P1) gene family have been identified (Klrb1a/NKR-P1A, Klrb1b/NKR-P1B, and Klrb1c/NKR-P1C), but in the human gene family, a single homologue has been designated KLRB1, NKR-P1A, or CD161. The KLRB1/NKR-P1 family of proteins are type-II-transmembrane C-type lectin receptors. KLRB1C/NKR-P1C activates NK-cell cytotoxicity, while KLRB1B/NKR-P1B functions as an inhibitory receptor. KLRB1B/NKR-P1B protein has intracellular Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM), while KLRB1C/NKR-P1C lacks ITIM and activates via association with Fc Receptor γ chain. Strikingly, KLRB1B/NKR-P1B and KLRB1C/NKR-P1C share 96 % amino acid sequence identity in their extracellular C-type lectin domains. The PK136 antibody Negative Control Purified NK-1.1 reacts with the NK-1.1 surface antigen encoded by the Klrb1c/NKR-P1C gene expressed on natural killer (NK) cells in selected strains of mice (eg, C57BL, FVB/N, NZB, but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, NOD, SJL, 129) and the antigen encoded by the Klrb1b/NKR-P1B gene expressed only on Swiss NIH and SJL mice, but not on C57BL/6. Expression of KLRB1C/NKR-P1C protein is correlated with the ability to lyse tumor cells in vitro and to mediate rejection of bone marrow allografts. The NK-1.1 marker is useful in defining NK cells, however, the antigen is also expressed on a rare, specialized population of T lymphocytes (NK-T cells) and some cultured monocytes. Plate-bound PK136 mAb, in combination with low concentrations of IL-2, induces proliferation of a subset of NK cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two color analysis of NK-1.1 expression on splenocytes. C57BL/6NHsd splenocytes were incubated simultaneously with PE-conjugated anti-mouse CD3e mAb 145-2C11 (Cat. No. 553063/553064) and purified mAb PK136 (right panel), followed by FITC-conjugated anti-mouse IgG2a mAb R19-15 (Cat. No. 553390). NK-1.1+ CD3e- NK cells and NK-1.1[dim] CD3e+ NK-T cells are detected. Flow cytometry was performed on a BD FACScan™ flow cytometry system. Page 1 of 2553162 Rev. 18

BD Pharmingen™ Purified Mouse Anti-Mouse NK-1.1 - Purified - Clone PK136 - Isotype Mouse IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse NK-1+ Spleen and Bone Marrow

Clone: PK136

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-NK-1.1/CD161c antibody (ABIN2689857)

Carlyle, Martin, Mehra, Attisano, Tsui, Zúñiga-Pflücker: "Mouse NKR-P1B, a novel NK1.1 antigen with inhibitory function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 5917-23, (1999) (PubMed).

Kung, Su, Shannon, Miller: "The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 10, pp. 5876-87, (1999) (PubMed).

Arase, Arase, Park, Ohno, Ra, Saito: "Association with FcRgamma is essential for activation signal through NKR-P1 (CD161) in natural killer (NK) cells and NK1.1+ T cells." in: The Journal of experimental medicine, Vol. 186, Issue 12, pp. 1957-63, (1998) (PubMed).

Reichlin, Yokoyama: "Natural killer cell proliferation induced by anti-NK1.1 and IL-2." in: Immunology and cell biology, Vol. 76, Issue 2, pp. 143-52, (1998) (PubMed).

Lanier: "Natural killer cells: from no receptors to too many." in: Immunity, Vol. 6, Issue 4, pp. 371-8, (1997) (PubMed).

Vicari, Zlotnik: "Mouse NK1.1+ T cells: a new family of T cells." in: Immunology today, Vol. 17, Issue 2, pp. 71-6, (1996) (PubMed).

Yokoyama, Seaman: "The Ly-49 and NKR-P1 gene families encoding lectin-like receptors on natural killer cells: the NK gene complex." in: Annual review of immunology, Vol. 11, pp. 613-35, (1993) (PubMed).

Yu, Kumar, Bennett: "Murine natural killer cells and marrow graft rejection." in: Annual review of immunology, Vol. 10, pp. 189-213, (1992) (PubMed).

Giorda, Trucco: "Mouse NKR-P1. A family of genes selectively coexpressed in adherent lymphokine-activated killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 5, pp. 1701-8, (1991) (PubMed).

Sentman, Hackett, Moore, Tutt, Bennett, Kumar: "Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen." in: Hybridoma, Vol. 8, Issue 6, pp. 605-14, (1990) (PubMed).

Sentman, Kumar, Koo, Bennett: "Effector cell expression of NK1.1, a murine natural killer cell-specific molecule, and ability of mice to reject bone marrow allografts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 6, pp. 1847-53, (1989) (PubMed).

Koo, Dumont, Tutt, Hackett, Kumar: "The NK-1.1(-) mouse: a model to study differentiation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 12, pp. 3742-7, (1987) (PubMed).

Koo, Peppard: "Establishment of monoclonal anti-Nk-1.1 antibody." in: Hybridoma, Vol. 3, Issue 3, pp. 301-3, (1985) (PubMed).

Target Details for NK-1.1/CD161c

Target: NK-1.1/CD161c (NK-1.1)

Abstract: NK-1.1/CD161c Products

Background: Synonyms: NKR-P1B and NKR-P1C