Pan Carcinoma antibody

Catalog No. ABIN2689873

Cited in 4+ publications. The Mouse Monoclonal anti-Pan Carcinoma antibody (Clone KS1-4) (ABIN2689873) specifically detects Pan Carcinoma in IHC (fro), FACS, IF and IP. The antibody is reactive with Human samples.

Quick Overview for Pan Carcinoma antibody (ABIN2689873)

Target: Pan Carcinoma

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: KS1-4

Product Details

Characteristics: KS1/4 antigen (Ag), defined by the KS1/4 antibody (KS1/4), is expressed in many epithelial-derived carcinomas and normal epithelial cell surfaces. The cDNA encoding KS1/4 has been isolated and contains an open reading frame of 314 amino acids including a putative signal sequence. KS1/4 Ag migrates as three glycosylated polypeptides with molecular masses of 35, 40 and 42 kDa. The 40 and 42 kDa species are similar proteins and appear to differ only by their degree of N-linked glycosylation. The 35 kDa species results from proteolytic cleavages of the larger molecular weight proteins. KS1/4 was first described as a monoclonal antibody which recognized a lung adenocarcinoma associated antigen. In oncolytic drug targeting studies, KS1/4 suppressed the growth of human lung tumor xenografts in athymic nude mice. Subsequent studies showed that KS1/4 reacts with a variety of tumor tissues including colon, breast, ovarian, and pancreas. It also recognizes normal epithelium including colon, stomach, small intestine, liver, kidney, lung, pancreas, skin and ovary. These results suggest that KS1/4 Ag represents an epithelial cell/epithelial-derived carcinoma marker. KS1/4 recognizes a 40 - 42 kDa antigen expressed on the cell surfaces of a variety of epithelial tumors and normal epithelial cell types. KS1/4 also recognizes a 35 kDa proteolytic fragment. UCLA-P3 cells derived from a human adenocarcinoma of the lung were used as immunogen. Profile of UCLA-P3 lung adenocarcinoma cells analyzed by flow cytometry. Cells were incubated with either KS1/4 (Cat. No. 554276) or an isotype control, followed by a FITC-conjugated second step antibody.

BD Pharmingen™ Purified Mouse Anti-40 kDa Pan Carcinoma - Purified - Clone KS1/4 - Isotype Mouse IgG2a - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: UCLA-P3 cells

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Perez, Walker: "Isolation and characterization of a cDNA encoding the KS1/4 epithelial carcinoma marker." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 10, pp. 3662-7, (1989) (PubMed).

Bumol, Marder, DeHerdt, Borowitz, Apelgren: "Characterization of the human tumor and normal tissue reactivity of the KS1/4 monoclonal antibody." in: Hybridoma, Vol. 7, Issue 4, pp. 407-15, (1988) (PubMed).

Spearman, Goodwin, Apelgren, Bumol: "Disposition of the monoclonal antibody-vinca alkaloid conjugate KS1/4-DAVLB (LY256787) and free 4-desacetylvinblastine in tumor-bearing nude mice." in: The Journal of pharmacology and experimental therapeutics, Vol. 241, Issue 2, pp. 695-703, (1987) (PubMed).

Varki, Reisfeld, Walker: "Antigens associated with a human lung adenocarcinoma defined by monoclonal antibodies." in: Cancer research, Vol. 44, Issue 2, pp. 681-7, (1984) (PubMed).

Target Details

Target: Pan Carcinoma