PIR-A/B (Extracellular Domain) antibody

Catalog No. ABIN2689880

This Rat Monoclonal antibody specifically detects PIR-A/B in FACS and IP. It exhibits reactivity toward Mouse. It has been mentioned in 6+ publications

Quick Overview for PIR-A/B (Extracellular Domain) antibody (ABIN2689880)

Target: PIR-A/B

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 6C1

Product Details

Binding Specificity: Extracellular Domain

Characteristics: The 6C1 antibody reacts with common epitopes of PIR-A and PIR-B (Paired Immunoglobulin-liked Receptors) in all mouse strains tested (A/J, BALB/cJ, C3H, C57BL/6, DBA/1, DBA/2, NZB, and SJL). PIR-A and PIR-B are type-I transmembrane glycoproteins containing six Ig-like domains. There are multiple PIR-A proteins which are activating receptors by virtue of their intracellular association with the ITAM (Immunoreceptor Tyrosine-based Activation Motif)- containing Fc receptor γ chain (FcRγ) in mast cells and macrophages. FcRγ expression is required for PIR-A cell-surface expression on dendritic cells, B cells, and myeloid lineages. In contrast, PIR-B is an inhibitory receptor which contains various ITIMs (Immunoreceptor Tyrosine-based Inhibitory Motifs) in its cytoplasmic domain. These receptors are expressed on B cells, granulocytes, mast cells, dendritic cells, and monocytes/macrophages, but not on thymocytes, T lymphocytes, erythroid lineage cells, or NK cells. The level of cell-surface expression of these receptors increases as a function of B-cell activation and myeloid- and B-lineage differentiation. The 6C1 antibody immunoprecipitates molecules of 85- and 125- kDa, which correspond to PIRA and PIR-B, respectively. The expression of PIR-A/B on mouse spleen cells. BALB/c splenocytes were preincubated with Mouse BD Fc Block™ (Cat. no. 553141/553142) and stained with purified 6C1 monoclonal antibody (shaded histogram) followed by biotin-conjugated RG11/39.4 mAb (anti-rat IgG1, Cat. no. 553890, both histograms), then Streptavidin-PE (Cat. no. 554061, both histograms). Flow cytometry was performed on a BD FACScan™

BD Pharmingen™ Purified Rat Anti-Mouse PIR-A/B - Purified - Clone 6C1 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant mouse PIR-A1 extracellular domain peptide and the mouse myeloid cell line, WEH13

Isotype: IgG1 kappa

Application Details

Application Notes: Flow Cytometry System.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Chen, Hurez, Brockenbrough, Kubagawa, Cooper: "Paternal monoallelic expression of the paired immunoglobulin-like receptors PIR-A and PIR-B." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6868-72, (1999) (PubMed).

Taylor, McVicar: "Functional association of FcepsilonRIgamma with arginine(632) of paired immunoglobulin-like receptor (PIR)-A3 in murine macrophages." in: Blood, Vol. 94, Issue 5, pp. 1790-6, (1999) (PubMed).

Kubagawa, Chen, Ho, Shimada, Gartland, Mashburn, Uehara, Ravetch, Cooper: "Biochemical nature and cellular distribution of the paired immunoglobulin-like receptors, PIR-A and PIR-B." in: The Journal of experimental medicine, Vol. 189, Issue 2, pp. 309-18, (1999) (PubMed).

Bléry, Kubagawa, Chen, Vély, Cooper, Vivier: "The paired Ig-like receptor PIR-B is an inhibitory receptor that recruits the protein-tyrosine phosphatase SHP-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 5, pp. 2446-51, (1998) (PubMed).

Maeda, Kurosaki, Kurosaki: "Paired immunoglobulin-like receptor (PIR)-A is involved in activating mast cells through its association with Fc receptor gamma chain." in: The Journal of experimental medicine, Vol. 188, Issue 5, pp. 991-5, (1998) (PubMed).

Kubagawa, Burrows, Cooper: "A novel pair of immunoglobulin-like receptors expressed by B cells and myeloid cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 5261-6, (1997) (PubMed).

Target Details

Target: PIR-A/B