Pre-B Cell Receptor antibody (Biotin)

Catalog No. ABIN2689881

There are 6+ publications for this product available. The Rat Monoclonal anti-Pre-B Cell Receptor antibody is suitable to detect Pre-B Cell Receptor in samples from Mouse. It has been validated for FACS.

Quick Overview for Pre-B Cell Receptor antibody (Biotin) (ABIN2689881)

Target: Pre-B Cell Receptor

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Clone: SL156

Product Details

Characteristics: The pre-B cell receptor (pre-BCR) expressed during the early stages of B lymphocyte development is a heterodimer of immunoglobulin heavy chain (IgH) with surrogate light chain, which is an Ig-light-chain-like molecule composed of the non-covalently linked Lambda 5 (λ5) and VpreB proteins. The pre-BCR is believed to control IgH repertoire selection and proliferation of differentiating B lymphocytes. The SL156 antibody reacts with a conformational epitope of the pre-BCR in transfected X63-Ag8.653 cells but not with surrogate light chain, λ5, or VpreB in the absence of IgH. It detects pre-BCR on pre-B cell lines, but not on pro-B cell lines or IgM-positive splenocytes. It also detects pre-BCR on the surface of pre-B cells, but not on Ig light chain (IgL)-positive B lymphocytes, from the bone marrow of normal mice. It has been noted that the cell-surface expression of pre-BCR is upregulated after a one-hour incubation of bone-marrow leukocytes in tissue culture medium at 37 °C. After immunization, cell-surface pre-BCR is detected on a subset of splenic IgL+ germinal-center B lymphocytes. Two-color analysis of Pre-B cell receptor (Pre-BCR) expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgM[b] mAb AF6-78 (Cat. No. 553519), C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37 °C to enhance Pre-BCR expression. Then the leukocytes were stained with either biotinylated rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553928, left panel) or biotinylated mAb SL156 (right panel) in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. No. 553141/553142), followed by Streptavidin-APC (Cat. No. 554067) and FITC-conjugated anti-mouse CD45R/B220 mAb (Cat. No. 553087/553088). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Biotin Rat Anti-Mouse Pre-B Cell Receptor - Biotin - Clone SL156 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified soluble complex of recombinant mu IgH, lambda5, and VpreB

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Shimizu, Mundt, Licence, Melchers, Mårtensson: "VpreB1/VpreB2/lambda 5 triple-deficient mice show impaired B cell development but functional allelic exclusion of the IgH locus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 12, pp. 6286-93, (2002) (PubMed).

Melchers, ten Boekel, Seidl, Kong, Yamagami, Onishi, Shimizu, Rolink, Andersson: "Repertoire selection by pre-B-cell receptors and B-cell receptors, and genetic control of B-cell development from immature to mature B cells." in: Immunological reviews, Vol. 175, pp. 33-46, (2000) (PubMed).

Mårtensson, Ceredig: "Review article: role of the surrogate light chain and the pre-B-cell receptor in mouse B-cell development." in: Immunology, Vol. 101, Issue 4, pp. 435-41, (2000) (PubMed).

Meffre, Papavasiliou, Cohen, de Bouteiller, Bell, Karasuyama, Schiff, Banchereau, Liu, Nussenzweig: "Antigen receptor engagement turns off the V(D)J recombination machinery in human tonsil B cells." in: The Journal of experimental medicine, Vol. 188, Issue 4, pp. 765-72, (1998) (PubMed).

Winkler, Rolink, Melchers, Karasuyama: "Precursor B cells of mouse bone marrow express two different complexes with the surrogate light chain on the surface." in: European journal of immunology, Vol. 25, Issue 2, pp. 446-50, (1995) (PubMed).

Karasuyama, Rolink, Melchers: "A complex of glycoproteins is associated with VpreB/lambda 5 surrogate light chain on the surface of mu heavy chain-negative early precursor B cell lines." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 469-78, (1993) (PubMed).

Target Details

Target: Pre-B Cell Receptor

Background: Synonyms: Pre-BCR