PTF1A antibody

Catalog No. ABIN2689886

This Mouse Monoclonal antibody specifically detects PTF1A in WB, IF, BI, IHC (f) and ICS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PTF1A antibody (ABIN2689886)

Target: PTF1A (Pancreas Specific Transcription Factor, 1a (PTF1A))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PTF1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS)

Clone: S25

Product Details anti-PTF1A Antibody

Brand: BD Pharmingen™

Characteristics: The S25-763 monoclonal antibody specifically binds PTF1A (Pancreas specific transcription factor 1a). PTF1A is a transcription factor that contains a basic helix-loop-helix motif domain and, in combination with p75 and p64, forms the pancreas transcription factor complex. PTF1A plays a roles in pancreatic and cerebellar development. In early pancreatic development, pancreatic bud cells can either continue to pancreatic organogenesis or regress to a duodenal fate, and PTF1A plays a role in this differentiation. In late pancreatic development, the expression of PTF1A is restricted to acinar cells where it is involved in the maintenance of exocrine-specific gene expression. PTF1A expression is often evaluated to monitor pancreatic lineage differentiation from pluripotent stem cells. In addition, PTF1A is expressed during retinal development, and mutations in PTF1A cause cerebellar deficiencies. This mouse anti-human PTF1A monoclonal antibody, clone S25-763, has been demonstrated to cross-react with rat and mouse pancreatic acinar cells. Analysis of PTF1A expression in rat pancreatic acinar cells by western blot and flow cytometry. Left panel: Lysates of the AR42J cell line (ATCC CRL-1492) were electrophoresed (SDS-PAGE) in a 4-20 % Tris-Glycine polyacrylamide gel, transferred to PVDF membranes and then probed with 2 (lane 1), 1 (lane 2), 0.5 (lane 3), 0.25 (lane 4) mg/mL of Purified Mouse anti-PTF1A. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat. No. 554002). Right panel: AR42J cells were fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050) and stained with matching concentrations of either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121, dashed line) or Purified Mouse anti-PTF1A monoclonal antibody (solid line). The second-step reagent was PE Goat Anti-Mouse Ig (550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact AR42J cells. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System. Immunohistochemical analysis of PTF1A expression by acinar cells within the human pancreas. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No. 550878, left panel) or Purified Mouse anti-PTF1A antibody (right panel). A three-step staining procedure that employs Biotin Goat Anti-Mouse Ig (Cat. No. 550337), Streptavidin- HRP (Cat. No. 550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. The acinar cell nuclei and cytoplasm stained positively for the expression of PTF1A. Original magnification: 40x.

BD Pharmingen™ Purified Mouse Anti-PTF1A - Purified - Clone S25-763 - Isotype Mouse IgG1, κ - Reactivity Rat, Hu, Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PTF1A Recombinant Protein

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

Target Details for PTF1A

Target: PTF1A (Pancreas Specific Transcription Factor, 1a (PTF1A))

Alternative Name: PTF1A

Background: Synonyms: PTF1α, Ptf1a, Ptf1α, bHLHa29, PTF1-p48

Molecular Weight: 48 kDa

Pathways: Retinoic Acid Receptor Signaling Pathway