RORC antibody

Catalog No. ABIN2689894

Cited in 9+ publications. The Mouse Monoclonal anti-RORC antibody (Clone Q31) (ABIN2689894) specifically detects RORC in WB, IHC (p), IHC (fro), ICS and IHC (f). The antibody is reactive with Human and Mouse samples.

Quick Overview for RORC antibody (ABIN2689894)

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RORC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Staining (ICS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: Q31

Product Details anti-RORC Antibody

Characteristics: The Q31-378 monoclonal antibody recognizes RORgamma t (RORγt), an isoform of RORgamma (RORγ). RORγt is a DNA-binding transcription factor that belongs to the ROR/RZR orphan nuclear receptor family. RORγt is expressed exclusively by lymphoid cells including CD4+CD8+ thymocytes, peripheral CD4+ Th17 and CD8+ Tc17 cells, NKT cells and innate lymphoid cells such as lymphoid tissue inducer (LTi) cells. RORγt plays essential roles in thymopoiesis, T cell homeostasis, differentiation of effector T lymphocytes and the development of secondary lymphoid tissues including lymph nodes and Peyer's patches. Analyses of RORγt Expression by Western bloting and Immunohistochemistry. Left Panel: Western blot analyses of RORγt expression by mouse splenocytes and thymocytes. Cell lysates from untreated mouse splenocytes (Spl, Lanes 1,2) and thymocytes (Thy, Lanes 3,4) (20 μg total cellular protein/lane) were electrophoresed (SDS-PAGE) and transferred to membranes. They were then probed with Purified Anti-Actin antibody (Cat. No. 612656/612657, Lanes 1,3 at 1 μg of antibody/mL) or Purified Mouse Anti-Mouse RORγt antibody (

BD Pharmingen™ Purified Mouse anti-Mouse RORγt - Purified - Clone Q31-378 - Isotype Mouse IgG2a, κ - Reactivity Ms, Hu - 50 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse RORgammat Recombinant Protein

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-RORC antibody (ABIN2689894)

Unutmaz: "RORC2: the master of human Th17 cell programming." in: European journal of immunology, Vol. 39, Issue 6, pp. 1452-5, (2009) (PubMed).

Hamada, Garcia-Hernandez, Reome, Misra, Strutt, McKinstry, Cooper, Swain, Dutton: "Tc17, a unique subset of CD8 T cells that can protect against lethal influenza challenge." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 6, pp. 3469-81, (2009) (PubMed).

Bechtel, Rosenfelder, Duda, Schmidt, Ernst, Wellenreuther, Mehrle, Schuster, Bahr, Blöcker, Heubner, Hoerlein, Michel, Wedler, Köhrer, Ottenwälder, Poustka, Wiemann, Schupp: "The full-ORF clone resource of the German cDNA Consortium." in: BMC genomics, Vol. 8, pp. 399, (2008) (PubMed).

Ivanov, McKenzie, Zhou, Tadokoro, Lepelley, Lafaille, Cua, Littman: "The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells." in: Cell, Vol. 126, Issue 6, pp. 1121-33, (2006) (PubMed).

Eberl, Littman: "The role of the nuclear hormone receptor RORgammat in the development of lymph nodes and Peyer's patches." in: Immunological reviews, Vol. 195, Issue 9, pp. 81-90, (2003) (PubMed).

Eberl, Marmon, Sunshine, Rennert, Choi, Littman: "An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells." in: Nature immunology, Vol. 5, Issue 1, pp. 64-73, (2003) (PubMed).

He, Deftos, Ojala, Bevan: "RORgamma t, a novel isoform of an orphan receptor, negatively regulates Fas ligand expression and IL-2 production in T cells." in: Immunity, Vol. 9, Issue 6, pp. 797-806, (1999) (PubMed).

Medvedev, Yan, Hirose, Giguère, Jetten: "Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element." in: Gene, Vol. 181, Issue 1-2, pp. 199-206, (1997) (PubMed).

Hirose, Smith, Jetten: "ROR gamma: the third member of ROR/RZR orphan receptor subfamily that is highly expressed in skeletal muscle." in: Biochemical and biophysical research communications, Vol. 205, Issue 3, pp. 1976-83, (1995) (PubMed).

Target Details for RORC

Target: RORC (RAR-Related Orphan Receptor C (RORC))

Alternative Name: RORgammat

Background: Synonyms: RORγT, RORgt, RORgamma t, RORgammaT, Rorc2, Rorg, TOR, Thor, Nr1f3

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway