RT1-BB antibody

Catalog No. ABIN2689897

Product Details anti-RT1-BB Antibody

Target: RT1-BB (RT1 Class II, Locus Bb (RT1-BB))

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This RT1-BB antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR), Immunoaffinity Chromatography (IAC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Characteristics: The OX-6 antibody reacts with non-polymorphic determinants of the Rat MHC class II antigen, I-A equivalent. RT1B is found on peripheral B lymphocytes, thymic cortical epithelial and medullary reticular cells, small intestinal villus epithelium, epidermal Langerhans cells, dendritic cells, some tissue macrophage populations, peritoneal mast cells, and a subset of thymocytes, but not on peripheral T cells, erythrocytes, or microglia. The OX-6 mAb cross-reacts with mouse I-A[k] and I-A[s] alloantigens and with a major subset of splenocytes from NOD (I-A[g7]) mice. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Purified Mouse Anti-Rat RT1B - Purified - Clone OX-6 - Isotype Mouse IgG1, κ - Reactivity Rat, Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Ia-like Glycoproteins from Wistar Thymocytes

Clone: OX

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-RT1-BB antibody (ABIN2689897)

Damoiseaux, Yagita, Okumura, van Breda Vriesman: "Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells." in: Journal of leukocyte biology, Vol. 64, Issue 6, pp. 803-9, (1998) (PubMed).

Dick, Ford, Forrester, Sedgwick: "Flow cytometric identification of a minority population of MHC class II positive cells in the normal rat retina distinct from CD45lowCD11b/c+CD4low parenchymal microglia." in: The British journal of ophthalmology, Vol. 79, Issue 9, pp. 834-40, (1996) (PubMed).

Chen-Woan, Delaney, Fournier, Wakizaka, Murase, Fung, Starzl, Demetris: "In vitro characterization of rat bone marrow-derived dendritic cells and their precursors." in: Journal of leukocyte biology, Vol. 59, Issue 2, pp. 196-207, (1996) (PubMed).

Fox, Jewell, Whitacre: "Rat peritoneal mast cells present antigen to a PPD-specific T cell line." in: Cellular immunology, Vol. 158, Issue 1, pp. 253-64, (1994) (PubMed).

Neiss, Becker, Knop, Reske: "Modulation of MHC class II determinants on rat Langerhans cells during short term culture." in: Advances in experimental medicine and biology, Vol. 329, pp. 29-34, (1993) (PubMed).

Xia, Schneeberger, McCarthy, Kradin: "Accessory cells of the lung. II. Ia+ pulmonary dendritic cells display cell surface antigen heterogeneity." in: American journal of respiratory cell and molecular biology, Vol. 5, Issue 3, pp. 276-83, (1991) (PubMed).

Steiniger, Falk, Lohmüller, van der Meide: "Class II MHC antigens in the rat digestive system. Normal distribution and induced expression after interferon-gamma treatment in vivo." in: Immunology, Vol. 68, Issue 4, pp. 507-13, (1990) (PubMed).

Mayrhofer, Pugh, Barclay: "The distribution, ontogeny and origin in the rat of Ia-positive cells with dendritic morphology and of Ia antigen in epithelia, with special reference to the intestine." in: European journal of immunology, Vol. 13, Issue 2, pp. 112-22, (1983) (PubMed).

Fukumoto, McMaster, Williams: "Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus." in: European journal of immunology, Vol. 12, Issue 3, pp. 237-43, (1982) (PubMed).

McMaster, Williams: "Identification of Ia glycoproteins in rat thymus and purification from rat spleen." in: European journal of immunology, Vol. 9, Issue 6, pp. 426-33, (1980) (PubMed).

Target Details for RT1-BB

Target: RT1-BB (RT1 Class II, Locus Bb (RT1-BB))

Alternative Name: RT1B (RT1-BB Products)

Synonyms: RT1.B antibody, RT1 class II, locus Bb antibody, RT1-Bb antibody

Pathways: Production of Molecular Mediator of Immune Response