SLUG antibody

Catalog No. ABIN2689904

This Mouse Monoclonal antibody specifically detects SLUG in WB, IF, BI, IHC (f) and ICS. It exhibits reactivity toward Human and Mouse. It has been mentioned in 3+ publications

Quick Overview for SLUG antibody (ABIN2689904)

Target: SLUG (SNAI2) (Snail Family Zinc Finger 2 (SNAI2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLUG antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS)

Clone: S43

Product Details anti-SLUG Antibody

Characteristics: The S43-1259 monoclonal antibody specifically recognizes SNAI2, also named Slug, which is a zinc finger transcriptional repressor first recognized for its roles in embryogenesis. SNAI2/Slug and other members of the snail family of transcription factors inhibit the expression of E-cadherin, which plays important roles in cell adhesion and in the maintenance of tissue structure. SNAI2/Slug activation and E-cadherin inhibition induces epithelial-mesenchymal transition (EMT) at several stages of embryonic development and also contributes to the invasiveness of malignancies. Aberrant expression of SNAI2/Slug has also been linked to cancer stem cell formation, cell cycle regulation and apoptosis. SNAI2/Slug is a downstream effector of various signaling pathways, including Akt, Wnt and SCF/ckit signaling. Immunoflourescent, flow cytometric, western blot, and immunohistochemical analysis of SNAI2/Slug in human sarcoma cells. FAR LEFT: RH-30 rhabdomyosarcoma cells (DSMZ, ACC 489) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Perm/Wash™ Buffer (Cat No. 554723) and stained with Purified Mouse Anti-SNAI2/Slug (pseudo-colored green) at 1.2 μg/mL followed by Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies). Nuclear counter -staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software. Permeabilization with BD Phosflow™ Perm Buffer III (Cat. No. 558050) or 0.1 % Triton™ X-100 buffer is also suitable. MIDDLE LEFT: RH-30 cells were fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat No. 562574) and stained with either Purified Mouse IgG1 κ Isotype Control (Cat No. 554121, dashed line) or Purified Mouse Anti-SNAI2/Slug (solid line), followed by PE Goat Anti-Mouse Ig (Multiple Adsorption) (Cat No. 550589). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometry was performed using a BD LSRFortessa™ Cell Analyzer. MIDDLE RIGHT: Lysates of RH-30 cells were electrophoresed (SDS-PAGE), transferred to PVDF membranes and then probed with 125, 60, 30, 15 and 7 ng/mL of Purified Mouse Anti-SNAI2/Slug (lanes 1-5) and HRP goat anti-mouse Ig (Cat. No. 554002). FAR RIGHT: Following antigen retrieval with Retrievagen A Buffer (Cat. No. 550524), formalin-fixed paraffin sections of a human fibrosarcoma were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, left) or Purified Mouse Anti-SNAI2/Slug (right). Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 550337), Streptavidin HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) were used to develop the staining. Original magnification: 40x.

BD Pharmingen™ Purified Mouse Anti-SNAI2/Slug - Purified - Clone S43-1259 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 50 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human SNAI2 Recombinant Protein

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-SLUG antibody (ABIN2689904)

Carpenter, Paw, Dewhirst, Lo: "Akt phosphorylates and activates HSF-1 independent of heat shock, leading to Slug overexpression and epithelial-mesenchymal transition (EMT) of HER2-overexpressing breast cancer cells." in: Oncogene, Vol. 34, Issue 5, pp. 546-57, (2015) (PubMed).

Guo, Keckesova, Donaher, Shibue, Tischler, Reinhardt, Itzkovitz, Noske, Zürrer-Härdi, Bell, Tam, Mani, van Oudenaarden, Weinberg: "Slug and Sox9 cooperatively determine the mammary stem cell state." in: Cell, Vol. 148, Issue 5, pp. 1015-28, (2012) (PubMed).

Francí, Takkunen, Dave, Alameda, Gómez, Rodríguez, Escrivà, Montserrat-Sentís, Baró, Garrido, Bonilla, Virtanen, García de Herreros: "Expression of Snail protein in tumor-stroma interface." in: Oncogene, Vol. 25, Issue 37, pp. 5134-44, (2006) (PubMed).

Target Details for SLUG

Target: SLUG (SNAI2) (Snail Family Zinc Finger 2 (SNAI2))

Alternative Name: SNAI2/Slug

Background: Synonyms: Snai2, SLUG, Slugh, SLUGH

Molecular Weight: 30-34 kDa