TLR4 antibody

Catalog No. ABIN2689940

This Mouse Monoclonal antibody specifically detects TLR4 in FACS and BR. It exhibits reactivity toward Human and has been mentioned in 11+ publications.

Quick Overview for TLR4 antibody (ABIN2689940)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: HTA125

Product Details anti-TLR4 Antibody

Brand: BD Pharmingen™

Characteristics: The monoclonal antibody HTA125 reacts with the human Toll-like receptor 4 (TLR4). Toll-like receptors (TLR) are type I transmembrane proteins characterized by an extracellular domain containing leucine-rich repeats and a cytoplasmic domain similar to IL-1R family. In human, ten TLR have been identified so far. The TLR family acts as pattern-recognition receptor is the essential component of pathogen recognition and innate immunity. TLR4 has been identified as the receptor for Gram-negative bacterial LPS. TLR4 mRNA has been found in spleen, placenta, ovary, intestine and lung. Using HTA125, TLR4 protein expression has been detected in monocytes, B cells, and T cells in human peripheral blood. Human TLR4 gene has been mapped to chromosome 9q32. The immunogen used to generate HTA125 hybridoma was human TLR4-transfected Ba/F3 cell line. Detection of TLR4 expression on human peripheral monocytes by purified anti-human TLR4 antibody HTA125 (Cat. No. 551964). Human PBMC were stained with 0.5 μg of purified HTA125 using 3-step staining protocol outlined below and anti-human CD14-FITC (Cat. No. 555397) and analyzed with a BD FACScan™ Flow Cytometer (Becton Dickinson, San Jose, CA).

BD Pharmingen™ Purified Mouse Anti-Human Toll-Like Receptor 4 - Purified - Clone HTA125 - Isotype Mouse IgG2a, κ - Reactivity Hu - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TLR4-transfected Ba/F3 cell line

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-TLR4 antibody (ABIN2689940)

Paik, Schwabe, Bataller, Russo, Jobin, Brenner: "Toll-like receptor 4 mediates inflammatory signaling by bacterial lipopolysaccharide in human hepatic stellate cells." in: Hepatology (Baltimore, Md.), Vol. 37, Issue 5, pp. 1043-55, (2003) (PubMed).

Zarember, Godowski: "Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leukocytes in response to microbes, their products, and cytokines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 2, pp. 554-61, (2002) (PubMed).

Akira: "Toll-like receptors and innate immunity." in: Advances in immunology, Vol. 78, pp. 1-56, (2001) (PubMed).

Chuang, Ulevitch: "Identification of hTLR10: a novel human Toll-like receptor preferentially expressed in immune cells." in: Biochimica et biophysica acta, Vol. 1518, Issue 1-2, pp. 157-61, (2001) (PubMed).

Beutler: "Tlr4: central component of the sole mammalian LPS sensor." in: Current opinion in immunology, Vol. 12, Issue 1, pp. 20-6, (2000) (PubMed).

Akashi, Ogata, Kirikae, Kirikae, Kawasaki, Nishijima, Shimazu, Nagai, Fukudome, Kimoto, Miyake: "Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2." in: Biochemical and biophysical research communications, Vol. 268, Issue 1, pp. 172-7, (2000) (PubMed).

Chuang, Ulevitch: "Cloning and characterization of a sub-family of human toll-like receptors: hTLR7, hTLR8 and hTLR9." in: European cytokine network, Vol. 11, Issue 3, pp. 372-8, (2000) (PubMed).

Du, Poltorak, Wei, Beutler: "Three novel mammalian toll-like receptors: gene structure, expression, and evolution." in: European cytokine network, Vol. 11, Issue 3, pp. 362-71, (2000) (PubMed).

Shimazu, Akashi, Ogata, Nagai, Fukudome, Miyake, Kimoto: "MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4." in: The Journal of experimental medicine, Vol. 189, Issue 11, pp. 1777-82, (1999) (PubMed).

Takeuchi, Kawai, Sanjo, Copeland, Gilbert, Jenkins, Takeda, Akira: "TLR6: A novel member of an expanding toll-like receptor family." in: Gene, Vol. 231, Issue 1-2, pp. 59-65, (1999) (PubMed).

Rock, Hardiman, Timans, Kastelein, Bazan: "A family of human receptors structurally related to Drosophila Toll." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 2, pp. 588-93, (1998) (PubMed).

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: Toll-Like Receptor 4

Background: Synonyms: TLR4, CD284

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins