CRLF2 antibody

Catalog No. ABIN2689945

Product Details anti-CRLF2 Antibody

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRLF2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Neutralization (Neut)

Brand: BD Pharmingen™

Characteristics: The 1F11/TSLPR monoclonal antibody specifically binds to Thymic Stromal Lymphopoietin Receptor (TSLPR). TSLPR is a member of the hematopoietin receptor superfamily and is also known as Cytokine Receptor-like Factor 2 (CRL2, CRLF2Y, CRLF2). The functional TSLPR complex consists of two subunits, TSLPR and the alpha subunit of the Interleukin-7 Receptor (IL-7Rα). Analysis of the TSLPR reveals sequence similarity with the common cytokine receptor gamma chain (γc, CD132). Functional TSLPRs are expressed by epithelial cells and a variety of hematopoietic cell types, including thymocytes, T cells, B cells, natural killer T cells, monocytes, macrophages, basophils, and dendritic cells (DC). Recent studies indicate that TSLP can activate multiple STAT (Signal Transducer and Activator of Transcription) signaling proteins. TSLP enhances the maturation and viability of DC. It strongly induces DC expression of the CD40 and CD80 costimulatory molecules and chemokines, e.g., TARC (Thymus and activation-regulated chemokine, CCL17) that can attract Th2 effector cells. TSLP supports B cell development. TSLP costimulates the proliferation of naive T cells in the presence of mature DC. TSLP is also able to increase the sensitivity of T cell receptor-activated CD4+ T cells to low doses of IL-2. In the presence of TSLP, the acute myeloid leukemia-derived cell line, MUTZ-3, shows induced growth and reduced apoptosis. CRLF2 deregulated gene expression is thought to be involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia. The 1F11/TSLPR antibody is reportedly a neutralizing antibody.

BD Pharmingen™ Purified NA/LE Mouse Anti-Human TSLP Receptor - NA/LE - Clone 1F11/TSLPR (also known as 1F11 and AB81_85.1F11) - Isotype Mouse IgG1, κ - Reactivity Hu - 100 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TSLP Receptor Transfected Cell Line

Clone: 1F11-TSLPR

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Aqueous buffered solution containing no preservative.

Preservative: Without preservative

Handling Advice: This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CRLF2 antibody (ABIN2689945)

Ito, Liu, Arima: "Cellular and molecular mechanisms of TSLP function in human allergic disorders--TSLP programs the "Th2 code" in dendritic cells." in: Allergology international : official journal of the Japanese Society of Allergology, Vol. 61, Issue 1, pp. 35-43, (2012) (PubMed).

Ziegler: "Thymic stromal lymphopoietin and allergic disease." in: The Journal of allergy and clinical immunology, Vol. 130, Issue 4, pp. 845-52, (2012) (PubMed).

Pedroza-Gonzalez, Xu, Wu, Aspord, Tindle, Marches, Gallegos, Burton, Savino, Hori, Tanaka, Zurawski, Zurawski, Bover, Liu, Banchereau, Palucka: "Thymic stromal lymphopoietin fosters human breast tumor growth by promoting type 2 inflammation." in: The Journal of experimental medicine, Vol. 208, Issue 3, pp. 479-90, (2011) (PubMed).

Arima, Watanabe, Hanabuchi, Chang, Sun, Liu: "Distinct signal codes generate dendritic cell functional plasticity." in: Science signaling, Vol. 3, Issue 105, pp. ra4, (2010) (PubMed).

Lu, Wang, Wang, Arima, Hanabuchi, Liu: "TSLP and IL-7 use two different mechanisms to regulate human CD4+ T cell homeostasis." in: The Journal of experimental medicine, Vol. 206, Issue 10, pp. 2111-9, (2009) (PubMed).

Russell, Capasso, Vater, Akasaka, Bernard, Calasanz, Chandrasekaran, Chapiro, Gesk, Griffiths, Guttery, Haferlach, Harder, Heidenreich, Irving, Kearney, Nguyen-Khac, Machado, Minto, Majid, Moorman et al.: "Deregulated expression of cytokine receptor gene, CRLF2, is involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia. ..." in: Blood, Vol. 114, Issue 13, pp. 2688-98, (2009) (PubMed).

Rochman, Watanabe, Arima, Liu, Leonard: "Cutting edge: direct action of thymic stromal lymphopoietin on activated human CD4+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 11, pp. 6720-4, (2007) (PubMed).

Tedla, Bandeira-Melo, Tassinari, Sloane, Samplaski, Cosman, Borges, Weller, Arm: "Activation of human eosinophils through leukocyte immunoglobulin-like receptor 7." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 3, pp. 1174-9, (2003) (PubMed).

Quentmeier, Drexler, Fleckenstein, Zaborski, Armstrong, Sims, Lyman: "Cloning of human thymic stromal lymphopoietin (TSLP) and signaling mechanisms leading to proliferation." in: Leukemia, Vol. 15, Issue 8, pp. 1286-92, (2001) (PubMed).

Target Details for CRLF2

Target: CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))

Alternative Name: TSLP Receptor (CRLF2 Products)

Synonyms: CRLF2 antibody, CRL2 antibody, CRLF2Y antibody, TSLPR antibody, CRLM2 antibody, Ly114 antibody, Tpte2 antibody, Tslpr antibody, Crl2 antibody, cytokine receptor like factor 2 antibody, cytokine receptor-like factor 2 antibody, CRLF2 antibody, Crlf2 antibody

Background: Synonyms: CRL2, CRLF2, CRLF2Y, Cytokine receptor-like factor 2, IL-XR, TSLPR