T Cell Receptor (TCR) V beta 11 antibody (Biotin)

Catalog No. ABIN2689951

Cited in 5+ publications. The Biotin-conjugated Rat Monoclonal anti-T Cell Receptor (TCR) V beta 11 antibody (Clone RR3) (ABIN2689951) specifically detects T Cell Receptor (TCR) V beta 11 in FACS. The antibody is reactive with Mouse samples.

Quick Overview for T Cell Receptor (TCR) V beta 11 antibody (Biotin) (ABIN2689951)

Target: T Cell Receptor (TCR) V beta 11

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Clone: RR3

Product Details

Characteristics: The RR3-15 antibody reacts with the Vβ 11 T-Cell Receptor (TCR) of mice having the b haplotype (e.g., A, C57BL, C58, DBA/1) of the Tcrb gene complex. The Tcrb-V11 gene locus is deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) and c (e.g., RIII) haplotypes. Vβ TCR-bearing T lymphocytes are clonally eliminated in mice expressing I-E and superantigens encoded by Mtv-9 (Etc-1, Mls[f], Dvb11.2) and/or Mtv-11 (Mls[f], Dvb 11.2) proviruses (e.g., AKR, BALB/c, CBA/J, C3H, DBA/2), and they are incompletely eliminated in mice expressing I-E and Mtv-8 (Mls[f], Dvb 11.1) superantigen (e.g., A). Activation of Vβ 11 TCR-expressing T cells by these determinants is dependent upon presentation by I-E. The bacterial superantigen Staphylococcal enterotoxin A (SEA) also interacts with Vβ 11 TCR, and in vivo exposure to SEA causes activation and subsequent deletion of Vβ TCR-expressing lymphocytes. Plate-bound RR3-15 antibody activates Vβ 11 TCR-bearing T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of Vβ 11 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotin-conjugated RR3-15, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 536.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a FACScan™ .

BD Pharmingen™ Biotin Rat Anti-Mouse Vβ 11 T-Cell Receptor - Biotin - Clone RR3-15 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.25 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Cytolytic T-Cell

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

McCormack, Callahan, Kappler, Marrack: "Profound deletion of mature T cells in vivo by chronic exposure to exogenous superantigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 9, pp. 3785-92, (1993) (PubMed).

Gao, Kanagawa, Sprent: "Capacity of unprimed CD4+ and CD8+ T cells expressing V beta 11 receptors to respond to I-E alloantigens in vivo." in: The Journal of experimental medicine, Vol. 170, Issue 6, pp. 1947-57, (1990) (PubMed).

Bill, Kanagawa, Woodland, Palmer: "The MHC molecule I-E is necessary but not sufficient for the clonal deletion of V beta 11-bearing T cells." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1405-19, (1989) (PubMed).

Haqqi, Banerjee, Anderson, David: "RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes." in: The Journal of experimental medicine, Vol. 169, Issue 6, pp. 1903-9, (1989) (PubMed).

Behlke, Chou, Huppi, Loh: "Murine T-cell receptor mutants with deletions of beta-chain variable region genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 767-71, (1986) (PubMed).

Target Details

Target: T Cell Receptor (TCR) V beta 11

Alternative Name: Vbeta 11 T-Cell Receptor