T Cell Receptor (TCR) V beta 3 antibody (Biotin)

Catalog No. ABIN2689955

This Armenian Hamster Monoclonal antibody specifically detects T Cell Receptor (TCR) V beta 3 in FACS. It exhibits reactivity toward Mouse. It has been mentioned in 4+ publications

Quick Overview for T Cell Receptor (TCR) V beta 3 antibody (Biotin) (ABIN2689955)

Target: T Cell Receptor (TCR) V beta 3

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Clone: KJ25

Product Details

Characteristics: The KJ25 antibody reacts with the Vβ 3 T-cell Receptor (TCR) of strains having the a (e.g., C57BR, SJL), b (e.g., AKR, CBA/Ca, C57BL, DBA/1), and c (e.g., RIII) haplotypes of the Tcrb gene complex. Vβ 3 TCR-bearing T lymphocytes are clonally eliminated either completely or partially in mice expressing superantigens encoded by the Mtv-1 (Mls-4[a], Mls[c]), Mtv-3 (Mls[c]), Mtv-6 (Mls-3[a], Mls[c]), Mtv-13 (Mls-2[a], Mls[c]), Mtv-27, Mtv-44, and/or Mtv-MAI endogenous proviruses (e.g., A, BALB/c, CBA/J, C3H/He, DBA/2, NZB, NZW). Vβ 3 TCR-bearing T cells are activated by the superantigenic Staphylococcal Enterotoxins A and B. Activation or elimination of Vβ 3 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes. Two-color analysis of the expression of VfÀ 3 TCR on peripheral lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotinylated KJ25(Panel B), PE-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553048/553049, both panels), and PE-conjugated 53-6.7 (anti-mouse CD8a, Cat. no. 553032/553033, both panels) monoclonal antibodies, followed by Avidin-FITC (Cat. no. 554057, both panels). Flow cytometry was performed on a FACScan™ .

BD Pharmingen™ Biotin Hamster Anti-Mouse Vβ 3 T-Cell Receptor - Biotin - Clone KJ25 - Isotype Armenian Hamster IgG2, κ - Reactivity Ms - 0.25 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: alphabeta TCR purified from mouse T-cell hybridoma 2B4.6

Isotype: IgG2 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

McCormack, Callahan, Kappler, Marrack: "Profound deletion of mature T cells in vivo by chronic exposure to exogenous superantigen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 9, pp. 3785-92, (1993) (PubMed).

Tomonari, Fairchild, Rosenwasser: "Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection." in: Immunological reviews, Vol. 131, pp. 131-68, (1993) (PubMed).

Yuuki, Yoshikai, Kishihara, Iwasaki, Matsuzaki, Ogimoto, Nomoto: "Deletion of self-reactive T cells in nude mice grafted with neonatal allogeneic thymus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 2, pp. 474-9, (1990) (PubMed).

Pullen, Marrack, Kappler: "The T-cell repertoire is heavily influenced by tolerance to polymorphic self-antigens." in: Nature, Vol. 335, Issue 6193, pp. 796-801, (1988) (PubMed).

Target Details

Target: T Cell Receptor (TCR) V beta 3

Alternative Name: Vbeta 3 T-Cell Receptor