V beta 5.1,5.2 antibody (Biotin)

Catalog No. ABIN2689956

There are 8+ publications for this product available. The Mouse Monoclonal anti-V beta 5.1,5.2 antibody is suitable to detect V beta 5.1,5.2 in samples from Mouse. It has been validated for FACS.

Quick Overview for V beta 5.1,5.2 antibody (Biotin) (ABIN2689956)

Target: V beta 5.1,5.2

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: Flow Cytometry (FACS)

Clone: MR9

Product Details

Characteristics: The MR9-4 antibody reacts with the Vβ 5.1 and Vβ 5.2 T-cell Receptors of strains having the b haplotype (e.g., C57BL) of the Tcrb gene complex. These gene loci are deleted in mice having the a (e.g., C57BR, C57L, SJL, SWR) or c (e.g., RIII) Tcrb haplotype. Vβ5.1 and 5.2 TCR-bearing T lymphocytes are clonally eliminated, either completely or partially, in mice expressing I-E and superantigens encoded by the Mtv-1 (Mls-4a, Mlsc), Mtv-3 (Mlsc), Mtv-8 (Mlsf), Mtv-9 (Etc-1, Mlsf), Mtv-11 (Mlsf), Mtv-13 (Mls-2a, Mlsc), Mtv-27, Mtv44 , and/or Mtv-MAI endogenous provirus (e.g., A, AKR, BALB/c, C3H/He, C58, CBA/Ca, CBA/J, DBA/2, NZB, NZW). Activation of Vβ5 TCR-expressing T cells by this determinant is dependent upon presentation by I-E. Plate-bound MR9-4 antibody activates Vβ5.1 or 5.2 TCR-bearing T cells. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Two-color analysis of the expression of Vβ 5.1, 5.2 TCR on peripheral T lymphocytes. C57BL/6 lymph node cells were incubated simultaneously with biotin-conjugated MR9-4, PE-conjugated RM4-5 (anti-CD4, Cat. No. 553048/553049), and PE-conjugated 53-6.7 (anti-CD8a, Cat. No. 553032/553033) monoclonal antibodies, followed by Avidin-FITC (Cat. No. 554057). Flow cytometry was performed on a FACScan .

BD Pharmingen™ Biotin Mouse Anti-Mouse Vβ 5.1, 5.2 T-Cell Receptor - Biotin - Clone MR9-4 - Isotype Mouse IgG1, κ - Reactivity Ms - 0.25 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse T-Cell Hybridoma 2HB51.8

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Tomonari, Fairchild, Rosenwasser: "Influence of viral superantigens on V beta- and V alpha-specific positive and negative selection." in: Immunological reviews, Vol. 131, pp. 131-68, (1993) (PubMed).

Hügin, Vacchio, Morse: "A virus-encoded "superantigen" in a retrovirus-induced immunodeficiency syndrome of mice." in: Science (New York, N.Y.), Vol. 252, Issue 5004, pp. 424-7, (1991) (PubMed).

Woodland, Happ, Gollob, Palmer: "An endogenous retrovirus mediating deletion of alpha beta T cells?" in: Nature, Vol. 349, Issue 6309, pp. 529-30, (1991) (PubMed).

Utsunomiya, Kosaka, Kanagawa: "Differential reactivity of V beta 9 T cells to minor lymphocyte stimulating antigen in vitro and in vivo." in: European journal of immunology, Vol. 21, Issue 4, pp. 1007-11, (1991) (PubMed).

Bill, Kanagawa, Linten, Utsunomiya, Palmer: "Class I and class II MHC gene products differentially affect the fate of V beta 5 bearing thymocytes." in: The Journal of molecular and cellular immunology : JMCI, Vol. 4, Issue 5, pp. 269-79; discussion 279-80, (1990) (PubMed).

Woodland, Happ, Bill, Palmer: "Requirement for cotolerogenic gene products in the clonal deletion of I-E reactive T cells." in: Science (New York, N.Y.), Vol. 247, Issue 4945, pp. 964-7, (1990) (PubMed).

Haqqi, Banerjee, Anderson, David: "RIII S/J (H-2r). An inbred mouse strain with a massive deletion of T cell receptor V beta genes." in: The Journal of experimental medicine, Vol. 169, Issue 6, pp. 1903-9, (1989) (PubMed).

Behlke, Chou, Huppi, Loh: "Murine T-cell receptor mutants with deletions of beta-chain variable region genes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 3, pp. 767-71, (1986) (PubMed).

Target Details

Target: V beta 5.1,5.2

Alternative Name: Vbeta 5.1, 5.2 T-Cell Receptor