TCR V beta 4 antibody (Biotin)

Catalog No. ABIN2689960

Product Details anti-TCR V beta 4 Antibody (Biotin)

Target: TCR V beta 4

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TCR V beta 4 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Characteristics: The KT4 antibody reacts with the Vβ4 T-cell Receptor (TCR) of mice having the a (e.g., C57BR, C57L, SJL, SWR), b (e.g., A, AKR, BALB/c, CBA, C3H/He, C57BL, C58, DBA/1, DBA/2), and c (e.g., RIII) haplotypes of the Tcrb gene complex. Vβ4 TCR-bearing cells are among the CD4+ autoreactive T cells which induce autoimmune thyroiditis after elimination of regulatory (CD5-bright) T-cell subsets. Plate-bound KT4 antibody activates Vβ4 TCR-bearing T cells. Two-color analysis of the expression of Vb 4 TCR on peripheral T lymphocytes. SJL lymph node cells were incubated simultaneously with biotinylated KT4 (Panel B), PE-conjugated RM4-5 (anti-mouse CD4, Cat. no. 553048/553049, both panels), and PE-conjugated 53-6.7 (anti-mouse CD8a, Cat. no. 553032/553033, both panels) monoclonal antibodies, followed by Avidin-FITC (Cat. no. 554057, both panels). Flow cytometry was performed on a FACScan™ .

BD Pharmingen™ Biotin Rat Anti-Mouse Vβ4 T-Cell Receptor - Biotin - Clone KT4 - Isotype Rat IgG2b, κ - Reactivity Ms - 0.25 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: KT4 B10.D2 mouse T-cell clone I3

Clone: KT4

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-TCR V beta 4 antibody (Biotin) (ABIN2689960)

Katz, Benoist, Mathis: "T helper cell subsets in insulin-dependent diabetes." in: Science (New York, N.Y.), Vol. 268, Issue 5214, pp. 1185-8, (1995) (PubMed).

Sugihara, Fujiwara, Shearer: "Autoimmune thyroiditis induced in mice depleted of particular T cell subsets. Characterization of thyroiditis-inducing T cell lines and clones derived from thyroid lesions." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 2, pp. 683-94, (1993) (PubMed).

Tomonari, Lovering, Spencer: "Correlation between the V beta 4+ CD8+ T-cell population and the H-2d haplotype." in: Immunogenetics, Vol. 31, Issue 5-6, pp. 333-9, (1990) (PubMed).

Target Details for TCR V beta 4

Target: TCR V beta 4

Alternative Name: Vbeta4 T-Cell Receptor